Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1042 | 5' | -56 | NC_000924.1 | + | 34107 | 0.67 | 0.587746 |
Target: 5'- uAUCACCGGUUcUUGAUGCGA-UGAAu -3' miRNA: 3'- uUAGUGGCCAGcGGCUACGCUgACUU- -5' |
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1042 | 5' | -56 | NC_000924.1 | + | 16318 | 0.68 | 0.512325 |
Target: 5'- --cCAUCaGUUGCUGcgGCGGCUGAAa -3' miRNA: 3'- uuaGUGGcCAGCGGCuaCGCUGACUU- -5' |
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1042 | 5' | -56 | NC_000924.1 | + | 25751 | 0.69 | 0.460967 |
Target: 5'- --gCAUCGG-CGCCGGUGauucaGGCUGAAc -3' miRNA: 3'- uuaGUGGCCaGCGGCUACg----CUGACUU- -5' |
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1042 | 5' | -56 | NC_000924.1 | + | 24976 | 0.72 | 0.341788 |
Target: 5'- cAGUCACCGGUCaguGCCuuUGCGGCgGAu -3' miRNA: 3'- -UUAGUGGCCAG---CGGcuACGCUGaCUu -5' |
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1042 | 5' | -56 | NC_000924.1 | + | 25672 | 1.03 | 0.001992 |
Target: 5'- gAAUCACCGGUCGCCGAUGCGACUGAAc -3' miRNA: 3'- -UUAGUGGCCAGCGGCUACGCUGACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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