Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10424 | 3' | -57.5 | NC_002687.1 | + | 61050 | 0.69 | 0.854182 |
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Target: 5'- -----aCGuCUGCCGCAUCAUGgGGCa -3' miRNA: 3'- uacaugGC-GACGGUGUAGUGCgCCGc -5' |
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| 10424 | 3' | -57.5 | NC_002687.1 | + | 198149 | 0.69 | 0.846672 |
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Target: 5'- -gGUGCCuaUgGCCACGUuuucCGCGCGGUGc -3' miRNA: 3'- uaCAUGGcgA-CGGUGUA----GUGCGCCGC- -5' |
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| 10424 | 3' | -57.5 | NC_002687.1 | + | 65186 | 0.7 | 0.823111 |
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Target: 5'- -gGUACCGCUGUguCAUggaCAC-CGGCGa -3' miRNA: 3'- uaCAUGGCGACGguGUA---GUGcGCCGC- -5' |
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| 10424 | 3' | -57.5 | NC_002687.1 | + | 293037 | 0.7 | 0.789537 |
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Target: 5'- -gGUACCGCUGCCcCcUCuCGCGGa- -3' miRNA: 3'- uaCAUGGCGACGGuGuAGuGCGCCgc -5' |
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| 10424 | 3' | -57.5 | NC_002687.1 | + | 225214 | 0.71 | 0.735497 |
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Target: 5'- -cGUGCCgGCgccGCCGCGUCG-GCGGCu -3' miRNA: 3'- uaCAUGG-CGa--CGGUGUAGUgCGCCGc -5' |
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| 10424 | 3' | -57.5 | NC_002687.1 | + | 307889 | 1.06 | 0.006534 |
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Target: 5'- gAUGUACCGCUGCCACAUCACGCGGCGu -3' miRNA: 3'- -UACAUGGCGACGGUGUAGUGCGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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