Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10424 | 5' | -61.3 | NC_002687.1 | + | 227305 | 0.66 | 0.81983 |
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Target: 5'- cACCACCGGcaGCCgacGGagccgacGGGGCGACgcccGCg -3' miRNA: 3'- -UGGUGGUC--CGGa--CCa------CCCCGCUGa---UG- -5' |
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| 10424 | 5' | -61.3 | NC_002687.1 | + | 226798 | 0.67 | 0.778346 |
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Target: 5'- uGCCgguGCCGGuGCC-GGUGGcGGUGGCggugGCg -3' miRNA: 3'- -UGG---UGGUC-CGGaCCACC-CCGCUGa---UG- -5' |
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| 10424 | 5' | -61.3 | NC_002687.1 | + | 308845 | 0.69 | 0.668124 |
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Target: 5'- uGCCACCggAGGCCucccgcugcucggUGGUgGGGGCGuCaGCg -3' miRNA: 3'- -UGGUGG--UCCGG-------------ACCA-CCCCGCuGaUG- -5' |
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| 10424 | 5' | -61.3 | NC_002687.1 | + | 307502 | 0.74 | 0.391114 |
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Target: 5'- cCCACCAGGCCUGGUGGucccagucacgccGcGUGAUgugGCa -3' miRNA: 3'- uGGUGGUCCGGACCACC-------------C-CGCUGa--UG- -5' |
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| 10424 | 5' | -61.3 | NC_002687.1 | + | 307855 | 1.07 | 0.002587 |
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Target: 5'- gACCACCAGGCCUGGUGGGGCGACUACc -3' miRNA: 3'- -UGGUGGUCCGGACCACCCCGCUGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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