Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10425 | 3' | -58.5 | NC_002687.1 | + | 201870 | 0.73 | 0.62764 |
Target: 5'- uGGCUCAcgcGcGCUCGGGCGGGGCAGGcGg -3' miRNA: 3'- gUUGGGU---CaUGGGCCCGUCCCGUUC-C- -5' |
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10425 | 3' | -58.5 | NC_002687.1 | + | 200956 | 0.73 | 0.62764 |
Target: 5'- uGGCUCAugcGcGCUCGGGCGGGGCAGGcGg -3' miRNA: 3'- gUUGGGU---CaUGGGCCCGUCCCGUUC-C- -5' |
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10425 | 3' | -58.5 | NC_002687.1 | + | 285127 | 0.73 | 0.618083 |
Target: 5'- aAACaCCAuGUACCUGGGCAuuGGGCAAa- -3' miRNA: 3'- gUUG-GGU-CAUGGGCCCGU--CCCGUUcc -5' |
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10425 | 3' | -58.5 | NC_002687.1 | + | 308420 | 0.76 | 0.478901 |
Target: 5'- -cACCCGGcgACCCcGGCGGGGCGGGc -3' miRNA: 3'- guUGGGUCa-UGGGcCCGUCCCGUUCc -5' |
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10425 | 3' | -58.5 | NC_002687.1 | + | 308737 | 1.1 | 0.003598 |
Target: 5'- uCAACCCAGUACCCGGGCAGGGCAAGGa -3' miRNA: 3'- -GUUGGGUCAUGGGCCCGUCCCGUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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