Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
1043 | 3' | -56.9 | NC_000924.1 | + | 51216 | 0.66 | 0.68381 |
Target: 5'- cUGCCGUcgGCGcGuuGCCACgGGCAa- -3' miRNA: 3'- cAUGGCA--CGCaCuuCGGUGgCCGUgg -5' |
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1043 | 3' | -56.9 | NC_000924.1 | + | 51687 | 0.66 | 0.662369 |
Target: 5'- cUACCGUGCGcggaggUGAAGUCAggGGCAa- -3' miRNA: 3'- cAUGGCACGC------ACUUCGGUggCCGUgg -5' |
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1043 | 3' | -56.9 | NC_000924.1 | + | 38491 | 0.66 | 0.630028 |
Target: 5'- cGUGCCGgagaGUcUGAaaaAGCCGCCGGaGCUg -3' miRNA: 3'- -CAUGGCa---CGcACU---UCGGUGGCCgUGG- -5' |
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1043 | 3' | -56.9 | NC_000924.1 | + | 56214 | 0.66 | 0.630028 |
Target: 5'- cUGCUG-GCGUGAcaa-ACCGGCAUCa -3' miRNA: 3'- cAUGGCaCGCACUucggUGGCCGUGG- -5' |
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1043 | 3' | -56.9 | NC_000924.1 | + | 54231 | 0.66 | 0.62679 |
Target: 5'- aUACUGUGCG-GAAgGCCACCaauGGUgugaaacaugugguGCCg -3' miRNA: 3'- cAUGGCACGCaCUU-CGGUGG---CCG--------------UGG- -5' |
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1043 | 3' | -56.9 | NC_000924.1 | + | 12540 | 0.67 | 0.60846 |
Target: 5'- aGUGCCGUGC-UGAAGCCAaacaaCAUCa -3' miRNA: 3'- -CAUGGCACGcACUUCGGUggcc-GUGG- -5' |
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1043 | 3' | -56.9 | NC_000924.1 | + | 30046 | 0.69 | 0.472897 |
Target: 5'- -aGCCGUGaccaaacagGAuauAGCCACCgcuGGCACCg -3' miRNA: 3'- caUGGCACgca------CU---UCGGUGG---CCGUGG- -5' |
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1043 | 3' | -56.9 | NC_000924.1 | + | 53409 | 0.69 | 0.453308 |
Target: 5'- -aACCGUaguGCG-GAAGCCAaaGGCAgCCc -3' miRNA: 3'- caUGGCA---CGCaCUUCGGUggCCGU-GG- -5' |
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1043 | 3' | -56.9 | NC_000924.1 | + | 17029 | 0.7 | 0.415506 |
Target: 5'- -gGCCGUGgGUGGaaaaAGCCGCUGaGCAgUg -3' miRNA: 3'- caUGGCACgCACU----UCGGUGGC-CGUgG- -5' |
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1043 | 3' | -56.9 | NC_000924.1 | + | 51477 | 0.72 | 0.307009 |
Target: 5'- -cACCGguaCGUGAagGGCUACCGGCuCCu -3' miRNA: 3'- caUGGCac-GCACU--UCGGUGGCCGuGG- -5' |
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1043 | 3' | -56.9 | NC_000924.1 | + | 50236 | 0.75 | 0.199665 |
Target: 5'- aUGCCGUguuuuccugauaGCGUGAauAGCCGCCcuGCGCCg -3' miRNA: 3'- cAUGGCA------------CGCACU--UCGGUGGc-CGUGG- -5' |
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1043 | 3' | -56.9 | NC_000924.1 | + | 27850 | 1.11 | 0.000594 |
Target: 5'- gGUACCGUGCGUGAAGCCACCGGCACCu -3' miRNA: 3'- -CAUGGCACGCACUUCGGUGGCCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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