Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10433 | 5' | -55.3 | NC_002687.1 | + | 237496 | 0.66 | 0.985328 |
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Target: 5'- aAAACAUGgacGCCGUacucaGGCcgaccGCGGGGUUa -3' miRNA: 3'- -UUUGUACa--CGGUAg----CCG-----CGCCCUAGa -5' |
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| 10433 | 5' | -55.3 | NC_002687.1 | + | 110768 | 0.66 | 0.977414 |
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Target: 5'- ---uGUGUGCUGUCGaCGaCGGGAUUUg -3' miRNA: 3'- uuugUACACGGUAGCcGC-GCCCUAGA- -5' |
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| 10433 | 5' | -55.3 | NC_002687.1 | + | 328236 | 0.66 | 0.974266 |
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Target: 5'- -----cGUGCCAcacgagaucaaagaUCGGCGCGGGcagCa -3' miRNA: 3'- uuuguaCACGGU--------------AGCCGCGCCCua-Ga -5' |
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| 10433 | 5' | -55.3 | NC_002687.1 | + | 307736 | 0.69 | 0.925672 |
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Target: 5'- --cCAUG-GCCAUCGGCGaGGGcgCc -3' miRNA: 3'- uuuGUACaCGGUAGCCGCgCCCuaGa -5' |
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| 10433 | 5' | -55.3 | NC_002687.1 | + | 161470 | 0.69 | 0.925672 |
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Target: 5'- --uCAUG-GCCAcgCGGC-CGGGAUCg -3' miRNA: 3'- uuuGUACaCGGUa-GCCGcGCCCUAGa -5' |
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| 10433 | 5' | -55.3 | NC_002687.1 | + | 182760 | 0.74 | 0.705449 |
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Target: 5'- -uGCAUGUgGCCAUaaCGGCgGUGGGGUCa -3' miRNA: 3'- uuUGUACA-CGGUA--GCCG-CGCCCUAGa -5' |
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| 10433 | 5' | -55.3 | NC_002687.1 | + | 332135 | 1.05 | 0.011797 |
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Target: 5'- gAAACAUGUGCCAUCGGCGCGGGAUCUu -3' miRNA: 3'- -UUUGUACACGGUAGCCGCGCCCUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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