Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10437 | 3' | -54.3 | NC_002687.1 | + | 275599 | 0.66 | 0.989914 |
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Target: 5'- cUGGCUcuUUGcgCCG-CGCUCAUGCa- -3' miRNA: 3'- -AUCGGc-AACuaGGCaGCGAGUACGcu -5' |
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| 10437 | 3' | -54.3 | NC_002687.1 | + | 167512 | 0.66 | 0.988604 |
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Target: 5'- aGGCgCGgugUGAUCCGUUGCgcc-GCGGa -3' miRNA: 3'- aUCG-GCa--ACUAGGCAGCGaguaCGCU- -5' |
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| 10437 | 3' | -54.3 | NC_002687.1 | + | 122519 | 0.68 | 0.971808 |
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Target: 5'- -cGCCGUgGAUCCGguugaggaugagGCUCAUGCa- -3' miRNA: 3'- auCGGCAaCUAGGCag----------CGAGUACGcu -5' |
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| 10437 | 3' | -54.3 | NC_002687.1 | + | 235552 | 0.68 | 0.970149 |
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Target: 5'- aGGCCGUUG--CCGaaGCUC-UGCGAc -3' miRNA: 3'- aUCGGCAACuaGGCagCGAGuACGCU- -5' |
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| 10437 | 3' | -54.3 | NC_002687.1 | + | 281496 | 0.72 | 0.848975 |
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Target: 5'- gGGCUGUUGAUCuCGcuUCGCUguUGCGu -3' miRNA: 3'- aUCGGCAACUAG-GC--AGCGAguACGCu -5' |
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| 10437 | 3' | -54.3 | NC_002687.1 | + | 3062 | 1.07 | 0.011277 |
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Target: 5'- aUAGCCGUUGAUCCGUCGCUCAUGCGAc -3' miRNA: 3'- -AUCGGCAACUAGGCAGCGAGUACGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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