Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10437 | 5' | -54 | NC_002687.1 | + | 231687 | 0.66 | 0.991522 |
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Target: 5'- --gCGCAUgguUGGUGUUGGUagaagaaGGUGCGAu -3' miRNA: 3'- uaaGCGUG---GCCGCAACUAg------UCACGCU- -5' |
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| 10437 | 5' | -54 | NC_002687.1 | + | 4428 | 0.66 | 0.991522 |
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Target: 5'- --aCGCuCCGGUGcacGcgCAGUGCGGg -3' miRNA: 3'- uaaGCGuGGCCGCaa-CuaGUCACGCU- -5' |
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| 10437 | 5' | -54 | NC_002687.1 | + | 311436 | 0.66 | 0.991522 |
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Target: 5'- ---gGCGCUGGCGgccGAuuUCAGUGCu- -3' miRNA: 3'- uaagCGUGGCCGCaa-CU--AGUCACGcu -5' |
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| 10437 | 5' | -54 | NC_002687.1 | + | 164590 | 0.67 | 0.984161 |
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Target: 5'- -cUCgGCACCGGCGaaagacucgggGAUCAGagaGCGAc -3' miRNA: 3'- uaAG-CGUGGCCGCaa---------CUAGUCa--CGCU- -5' |
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| 10437 | 5' | -54 | NC_002687.1 | + | 214979 | 0.67 | 0.982694 |
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Target: 5'- cGUUCGaCACCGGCGggGccgaGGgcgGCGAc -3' miRNA: 3'- -UAAGC-GUGGCCGCaaCuag-UCa--CGCU- -5' |
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| 10437 | 5' | -54 | NC_002687.1 | + | 236858 | 0.68 | 0.976508 |
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Target: 5'- --aCGCGCCGGCGcacguauguggagaGAUUGGUGUGGc -3' miRNA: 3'- uaaGCGUGGCCGCaa------------CUAGUCACGCU- -5' |
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| 10437 | 5' | -54 | NC_002687.1 | + | 194483 | 0.7 | 0.942483 |
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Target: 5'- --gCGCAggcggaCGGCGUUGAcagCAGUGCGc -3' miRNA: 3'- uaaGCGUg-----GCCGCAACUa--GUCACGCu -5' |
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| 10437 | 5' | -54 | NC_002687.1 | + | 276145 | 0.75 | 0.747761 |
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Target: 5'- -gUCGCGCCGGCGUcGAUgAGccUGCGc -3' miRNA: 3'- uaAGCGUGGCCGCAaCUAgUC--ACGCu -5' |
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| 10437 | 5' | -54 | NC_002687.1 | + | 249107 | 0.76 | 0.66047 |
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Target: 5'- ---gGUACCGGCGUUGAagagcgUCGGUGUGGc -3' miRNA: 3'- uaagCGUGGCCGCAACU------AGUCACGCU- -5' |
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| 10437 | 5' | -54 | NC_002687.1 | + | 3096 | 1.07 | 0.012803 |
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Target: 5'- cAUUCGCACCGGCGUUGAUCAGUGCGAc -3' miRNA: 3'- -UAAGCGUGGCCGCAACUAGUCACGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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