Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10450 | 5' | -45.2 | NC_002687.1 | + | 281956 | 0.72 | 0.999788 |
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Target: 5'- ----cAGGGGCGgugCCGGGGgGCAAUCGc -3' miRNA: 3'- aaaauUCCUUGCa--GGUUCC-UGUUAGC- -5' |
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| 10450 | 5' | -45.2 | NC_002687.1 | + | 97050 | 0.72 | 0.999788 |
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Target: 5'- ---gAAGGAACGggagCCGAGGAUug-CGg -3' miRNA: 3'- aaaaUUCCUUGCa---GGUUCCUGuuaGC- -5' |
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| 10450 | 5' | -45.2 | NC_002687.1 | + | 30590 | 0.72 | 0.999659 |
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Target: 5'- ---cGGGGggUugCCGAGGACAGUCa -3' miRNA: 3'- aaaaUUCCuuGcaGGUUCCUGUUAGc -5' |
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| 10450 | 5' | -45.2 | NC_002687.1 | + | 239305 | 0.8 | 0.945992 |
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Target: 5'- ---gAAGGAGCGUCuCGAGGACGAguaCGa -3' miRNA: 3'- aaaaUUCCUUGCAG-GUUCCUGUUa--GC- -5' |
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| 10450 | 5' | -45.2 | NC_002687.1 | + | 22688 | 0.84 | 0.841558 |
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Target: 5'- cUUUUAAGGGACGUCCAA-GACgAAUCGg -3' miRNA: 3'- -AAAAUUCCUUGCAGGUUcCUG-UUAGC- -5' |
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| 10450 | 5' | -45.2 | NC_002687.1 | + | 22358 | 1.09 | 0.067112 |
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Target: 5'- cUUUUAAGGAACGUCCAAGGACAAUCGg -3' miRNA: 3'- -AAAAUUCCUUGCAGGUUCCUGUUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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