Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10468 | 3' | -56.8 | NC_002687.1 | + | 64958 | 0.66 | 0.967543 |
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Target: 5'- ---uGCUUGuGGCAgaacaGGGGUCGAGc -3' miRNA: 3'- gaccCGAGCuCCGUaaa--CCCCAGCUC- -5' |
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| 10468 | 3' | -56.8 | NC_002687.1 | + | 268103 | 0.67 | 0.950456 |
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Target: 5'- -gGGGCUgCGGGGgGgggGGGGagGGGg -3' miRNA: 3'- gaCCCGA-GCUCCgUaaaCCCCagCUC- -5' |
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| 10468 | 3' | -56.8 | NC_002687.1 | + | 126412 | 0.67 | 0.940885 |
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Target: 5'- -cGGGCUcaagaucagCGAGGCGUUcgacgcgaagaacuUGGGGaUGGGg -3' miRNA: 3'- gaCCCGA---------GCUCCGUAA--------------ACCCCaGCUC- -5' |
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| 10468 | 3' | -56.8 | NC_002687.1 | + | 86074 | 0.69 | 0.900653 |
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Target: 5'- -gGGGCcagCGGGGCcagUGGGGccagCGGGg -3' miRNA: 3'- gaCCCGa--GCUCCGuaaACCCCa---GCUC- -5' |
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| 10468 | 3' | -56.8 | NC_002687.1 | + | 304531 | 0.69 | 0.87485 |
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Target: 5'- -cGGGgUCGGGGUc---GGGGUCGGa -3' miRNA: 3'- gaCCCgAGCUCCGuaaaCCCCAGCUc -5' |
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| 10468 | 3' | -56.8 | NC_002687.1 | + | 64560 | 1.09 | 0.005249 |
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Target: 5'- cCUGGGCUCGAGGCAUUUGGGGUCGAGg -3' miRNA: 3'- -GACCCGAGCUCCGUAAACCCCAGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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