Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10469 | 3' | -51.6 | NC_002687.1 | + | 39371 | 0.73 | 0.935175 |
Target: 5'- aGcAUCAGCG-CUguuuuucCGCCGCCCUGg -3' miRNA: 3'- aC-UAGUCGCuGAaaua---GCGGUGGGAC- -5' |
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10469 | 3' | -51.6 | NC_002687.1 | + | 215360 | 0.73 | 0.924983 |
Target: 5'- -cGUCAucGUGACgaUGUCGCCGCCCUc -3' miRNA: 3'- acUAGU--CGCUGaaAUAGCGGUGGGAc -5' |
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10469 | 3' | -51.6 | NC_002687.1 | + | 200703 | 0.76 | 0.828699 |
Target: 5'- cGAauUCuGCGACUUUGUCGUCGCCaugCUGg -3' miRNA: 3'- aCU--AGuCGCUGAAAUAGCGGUGG---GAC- -5' |
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10469 | 3' | -51.6 | NC_002687.1 | + | 277196 | 0.79 | 0.659385 |
Target: 5'- uUGGUCuuGGCGACUUcGUCGUCGCUCUGu -3' miRNA: 3'- -ACUAG--UCGCUGAAaUAGCGGUGGGAC- -5' |
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10469 | 3' | -51.6 | NC_002687.1 | + | 65341 | 1.1 | 0.014432 |
Target: 5'- gUGAUCAGCGACUUUAUCGCCACCCUGa -3' miRNA: 3'- -ACUAGUCGCUGAAAUAGCGGUGGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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