Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10478 | 3' | -55.8 | NC_002687.1 | + | 223476 | 0.7 | 0.88891 |
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Target: 5'- gGCGgagguucgggauGCGGUGgcGG-GGGCGGGGGa- -3' miRNA: 3'- gCGU------------UGCCACuuCCaCUCGCUCCCac -5' |
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| 10478 | 3' | -55.8 | NC_002687.1 | + | 225628 | 0.71 | 0.853439 |
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Target: 5'- aCGCAcCGGUGGGcGGUcGAGCuggagccggacagGGGGGUGg -3' miRNA: 3'- -GCGUuGCCACUU-CCA-CUCG-------------CUCCCAC- -5' |
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| 10478 | 3' | -55.8 | NC_002687.1 | + | 226820 | 0.72 | 0.83113 |
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Target: 5'- gGUGGCGGUGgcGGUG-GCgGAGcGGUGa -3' miRNA: 3'- gCGUUGCCACuuCCACuCG-CUC-CCAC- -5' |
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| 10478 | 3' | -55.8 | NC_002687.1 | + | 89055 | 0.74 | 0.735497 |
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Target: 5'- gGUggUGGUGguGGUGucugucGCGGGGGUGa -3' miRNA: 3'- gCGuuGCCACuuCCACu-----CGCUCCCAC- -5' |
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| 10478 | 3' | -55.8 | NC_002687.1 | + | 172285 | 0.74 | 0.716751 |
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Target: 5'- uGCggUGGgGAAcGGUGggGGCGGGGGUGg -3' miRNA: 3'- gCGuuGCCaCUU-CCAC--UCGCUCCCAC- -5' |
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| 10478 | 3' | -55.8 | NC_002687.1 | + | 224163 | 0.75 | 0.659194 |
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Target: 5'- gCGCuGGCGGUGgcGGUG-GCGGGGGc- -3' miRNA: 3'- -GCG-UUGCCACuuCCACuCGCUCCCac -5' |
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| 10478 | 3' | -55.8 | NC_002687.1 | + | 224995 | 0.77 | 0.543368 |
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Target: 5'- aGCGGCGGUGGAGcuggagcugGAGCGGGuGGUGg -3' miRNA: 3'- gCGUUGCCACUUCca-------CUCGCUC-CCAC- -5' |
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| 10478 | 3' | -55.8 | NC_002687.1 | + | 83353 | 1.08 | 0.008503 |
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Target: 5'- uCGCAACGGUGAAGGUGAGCGAGGGUGu -3' miRNA: 3'- -GCGUUGCCACUUCCACUCGCUCCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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