Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1048 | 3' | -59.1 | NC_000924.1 | + | 26920 | 0.66 | 0.559767 |
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Target: 5'- -gGGGAUGGgGCuGGCCUgGCAAUCAc -3' miRNA: 3'- cgUCCUGCUgCG-UCGGGgCGUUGGUc -5' |
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| 1048 | 3' | -59.1 | NC_000924.1 | + | 56673 | 0.66 | 0.559767 |
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Target: 5'- aCGGGACGcCGuCAGgucaCCCGCcGCCAc -3' miRNA: 3'- cGUCCUGCuGC-GUCg---GGGCGuUGGUc -5' |
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| 1048 | 3' | -59.1 | NC_000924.1 | + | 25569 | 0.66 | 0.538973 |
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Target: 5'- cGCAGGACGuccuuCaCuGCCuCUGCAgugACCAGg -3' miRNA: 3'- -CGUCCUGCu----GcGuCGG-GGCGU---UGGUC- -5' |
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| 1048 | 3' | -59.1 | NC_000924.1 | + | 41283 | 0.67 | 0.507292 |
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Target: 5'- cCGGGAacugcgguacaacUGACGCuGGCUCCGguGCCAu -3' miRNA: 3'- cGUCCU-------------GCUGCG-UCGGGGCguUGGUc -5' |
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| 1048 | 3' | -59.1 | NC_000924.1 | + | 7121 | 0.71 | 0.270663 |
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Target: 5'- cGguGGugGGCGUugauggcuGGUCCCGCAucaucaacgaaaACCAGc -3' miRNA: 3'- -CguCCugCUGCG--------UCGGGGCGU------------UGGUC- -5' |
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| 1048 | 3' | -59.1 | NC_000924.1 | + | 50347 | 1.1 | 0.000382 |
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Target: 5'- aGCAGGACGACGCAGCCCCGCAACCAGa -3' miRNA: 3'- -CGUCCUGCUGCGUCGGGGCGUUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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