Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10489 | 3' | -55.7 | NC_002687.1 | + | 63691 | 0.66 | 0.979837 |
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Target: 5'- -cGUGGgCGGCggCUUCCCCuaugaugucGUGCg -3' miRNA: 3'- caCACCgGCUGuaGAAGGGGuc-------CAUG- -5' |
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| 10489 | 3' | -55.7 | NC_002687.1 | + | 203677 | 0.66 | 0.979837 |
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Target: 5'- -cGUGGCCGACuguGUCg-CCCCGGa--- -3' miRNA: 3'- caCACCGGCUG---UAGaaGGGGUCcaug -5' |
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| 10489 | 3' | -55.7 | NC_002687.1 | + | 61566 | 0.67 | 0.957285 |
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Target: 5'- cUGUGGCaUGACaAUCUUUUCUcGGUACa -3' miRNA: 3'- cACACCG-GCUG-UAGAAGGGGuCCAUG- -5' |
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| 10489 | 3' | -55.7 | NC_002687.1 | + | 325826 | 0.68 | 0.953585 |
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Target: 5'- -cGUGGCCGACA-CU-CCUguGGUc- -3' miRNA: 3'- caCACCGGCUGUaGAaGGGguCCAug -5' |
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| 10489 | 3' | -55.7 | NC_002687.1 | + | 146174 | 0.7 | 0.898623 |
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Target: 5'- --cUGGCCugu-UCUUCCgCCAGGUGCa -3' miRNA: 3'- cacACCGGcuguAGAAGG-GGUCCAUG- -5' |
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| 10489 | 3' | -55.7 | NC_002687.1 | + | 85475 | 0.7 | 0.892322 |
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Target: 5'- uUGUGGCCGACGUCUUUUUCAa---- -3' miRNA: 3'- cACACCGGCUGUAGAAGGGGUccaug -5' |
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| 10489 | 3' | -55.7 | NC_002687.1 | + | 266412 | 0.71 | 0.83471 |
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Target: 5'- ---aGGCCGggGCAUCa--CCCAGGUACg -3' miRNA: 3'- cacaCCGGC--UGUAGaagGGGUCCAUG- -5' |
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| 10489 | 3' | -55.7 | NC_002687.1 | + | 316775 | 0.72 | 0.810149 |
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Target: 5'- -cGUGGCaCGAC--CUUCCuCCAGGUAg -3' miRNA: 3'- caCACCG-GCUGuaGAAGG-GGUCCAUg -5' |
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| 10489 | 3' | -55.7 | NC_002687.1 | + | 99669 | 1.1 | 0.00533 |
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Target: 5'- cGUGUGGCCGACAUCUUCCCCAGGUACa -3' miRNA: 3'- -CACACCGGCUGUAGAAGGGGUCCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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