miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1050 3' -57.4 NC_000924.1 + 24597 0.66 0.626879
Target:  5'- -uGGAGUACGCCG-CGGGCaaCGGugcGGc -3'
miRNA:   3'- guCUUCAUGCGGCuGUCCG--GCCu--CCu -5'
1050 3' -57.4 NC_000924.1 + 26518 0.67 0.573313
Target:  5'- cCAGGAGagcGCGCUGGCGuGCUGG-GGAa -3'
miRNA:   3'- -GUCUUCa--UGCGGCUGUcCGGCCuCCU- -5'
1050 3' -57.4 NC_000924.1 + 6353 0.68 0.531235
Target:  5'- cCGGAAGUuaACGCUaaGGCucuGGCCuGGGGAa -3'
miRNA:   3'- -GUCUUCA--UGCGG--CUGu--CCGGcCUCCU- -5'
1050 3' -57.4 NC_000924.1 + 51279 0.69 0.45966
Target:  5'- gAGAAGUggcgcguACGCCGGaugaaaauCAGGCCGGAcguGGu -3'
miRNA:   3'- gUCUUCA-------UGCGGCU--------GUCCGGCCU---CCu -5'
1050 3' -57.4 NC_000924.1 + 51932 0.7 0.413365
Target:  5'- -cGAAG-ACGaaacacaauaCGGCAGGCUGGGGGGa -3'
miRNA:   3'- guCUUCaUGCg---------GCUGUCCGGCCUCCU- -5'
1050 3' -57.4 NC_000924.1 + 51590 1.08 0.000806
Target:  5'- gCAGAAGUACGCCGACAGGCCGGAGGAa -3'
miRNA:   3'- -GUCUUCAUGCGGCUGUCCGGCCUCCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.