Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1051 | 5' | -49.4 | NC_000924.1 | + | 13595 | 0.66 | 0.961986 |
Target: 5'- aGGAGCGUAAUGUgGCAgAUCuCGAu- -3' miRNA: 3'- gCCUUGCAUUGCG-CGUaUAGcGCUuu -5' |
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1051 | 5' | -49.4 | NC_000924.1 | + | 33445 | 0.69 | 0.879739 |
Target: 5'- gGGAGCGg--UGCGCuuauUCGCGggGg -3' miRNA: 3'- gCCUUGCauuGCGCGuau-AGCGCuuU- -5' |
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1051 | 5' | -49.4 | NC_000924.1 | + | 14370 | 0.7 | 0.836971 |
Target: 5'- gCGGAACagcACGCaCAUGUCGCGGu- -3' miRNA: 3'- -GCCUUGcauUGCGcGUAUAGCGCUuu -5' |
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1051 | 5' | -49.4 | NC_000924.1 | + | 53828 | 0.72 | 0.724597 |
Target: 5'- aCGGAugGUGACGUuauCAcUGUCGCGGGu -3' miRNA: 3'- -GCCUugCAUUGCGc--GU-AUAGCGCUUu -5' |
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1051 | 5' | -49.4 | NC_000924.1 | + | 54937 | 1.07 | 0.005827 |
Target: 5'- cCGGAACGUAACGCGCAUAUCGCGAAAa -3' miRNA: 3'- -GCCUUGCAUUGCGCGUAUAGCGCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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