Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10511 | 5' | -60.1 | NC_002687.1 | + | 27563 | 0.67 | 0.853183 |
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Target: 5'- cGACUCGCgCCGCucaGCgaccgUGUCGgGGGc- -3' miRNA: 3'- -CUGAGCGgGGCG---CGa----ACAGUgCCCua -5' |
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| 10511 | 5' | -60.1 | NC_002687.1 | + | 208435 | 0.68 | 0.81783 |
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Target: 5'- cGGgUCGUUCuCGCGCUUGaugggguucuuccaaUCGCGGGAc -3' miRNA: 3'- -CUgAGCGGG-GCGCGAAC---------------AGUGCCCUa -5' |
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| 10511 | 5' | -60.1 | NC_002687.1 | + | 57100 | 0.68 | 0.789674 |
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Target: 5'- cGACg-GCCCCGaCGCUagacccGUCaACGGGAUg -3' miRNA: 3'- -CUGagCGGGGC-GCGAa-----CAG-UGCCCUA- -5' |
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| 10511 | 5' | -60.1 | NC_002687.1 | + | 232623 | 0.68 | 0.789674 |
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Target: 5'- --gUCGCCCUG-GU-UGUCGCGGGAg -3' miRNA: 3'- cugAGCGGGGCgCGaACAGUGCCCUa -5' |
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| 10511 | 5' | -60.1 | NC_002687.1 | + | 111453 | 0.69 | 0.735779 |
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Target: 5'- uGugUCGCCCCGUGCUgacaccgGUCucugaaaACGaGGAc -3' miRNA: 3'- -CugAGCGGGGCGCGAa------CAG-------UGC-CCUa -5' |
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| 10511 | 5' | -60.1 | NC_002687.1 | + | 316245 | 0.72 | 0.576272 |
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Target: 5'- -cCUCGUCCCuCGCg-GUCACGGGAg -3' miRNA: 3'- cuGAGCGGGGcGCGaaCAGUGCCCUa -5' |
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| 10511 | 5' | -60.1 | NC_002687.1 | + | 108282 | 0.75 | 0.400204 |
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Target: 5'- aGGCUCGCUCCuuGUcgGUCACGGGAg -3' miRNA: 3'- -CUGAGCGGGGcgCGaaCAGUGCCCUa -5' |
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| 10511 | 5' | -60.1 | NC_002687.1 | + | 159628 | 1.04 | 0.005449 |
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Target: 5'- gGACUCGCCCCGCGCUUGUCACGGGAUc -3' miRNA: 3'- -CUGAGCGGGGCGCGAACAGUGCCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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