Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10526 | 3' | -55.8 | NC_002687.1 | + | 277002 | 0.66 | 0.978018 |
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Target: 5'- uUCGggGgaacaccGGGAuGUGUUCcaacAUGUGCACAu -3' miRNA: 3'- -AGCuuCa------CCCU-CGCGAG----UACGCGUGU- -5' |
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| 10526 | 3' | -55.8 | NC_002687.1 | + | 207673 | 0.67 | 0.954219 |
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Target: 5'- -aGGAGUGGGAGUGUggaGUGCGa--- -3' miRNA: 3'- agCUUCACCCUCGCGag-UACGCgugu -5' |
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| 10526 | 3' | -55.8 | NC_002687.1 | + | 265882 | 0.67 | 0.954219 |
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Target: 5'- uUCaAGGUGGGAGaaGCaUCAUGgGCGCu -3' miRNA: 3'- -AGcUUCACCCUCg-CG-AGUACgCGUGu -5' |
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| 10526 | 3' | -55.8 | NC_002687.1 | + | 199052 | 0.68 | 0.932715 |
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Target: 5'- gCGaAAGuUGGGGGCGa--GUGUGCGCAu -3' miRNA: 3'- aGC-UUC-ACCCUCGCgagUACGCGUGU- -5' |
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| 10526 | 3' | -55.8 | NC_002687.1 | + | 48133 | 0.7 | 0.893447 |
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Target: 5'- -aGAAGUGGGAGaGgUCuuugguUGUGCACAu -3' miRNA: 3'- agCUUCACCCUCgCgAGu-----ACGCGUGU- -5' |
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| 10526 | 3' | -55.8 | NC_002687.1 | + | 67605 | 0.7 | 0.880302 |
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Target: 5'- aUCGAGGaGGcGGCGCagUCGUGCGgACAg -3' miRNA: 3'- -AGCUUCaCCcUCGCG--AGUACGCgUGU- -5' |
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| 10526 | 3' | -55.8 | NC_002687.1 | + | 167301 | 0.7 | 0.873423 |
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Target: 5'- aCGAAGUcgGGGGGUGUguucCcgGCGUACAg -3' miRNA: 3'- aGCUUCA--CCCUCGCGa---GuaCGCGUGU- -5' |
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| 10526 | 3' | -55.8 | NC_002687.1 | + | 304434 | 0.72 | 0.777027 |
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Target: 5'- uUCGu-GUGGGAGCGC-CAUGCG-ACc -3' miRNA: 3'- -AGCuuCACCCUCGCGaGUACGCgUGu -5' |
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| 10526 | 3' | -55.8 | NC_002687.1 | + | 198673 | 1.08 | 0.006832 |
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Target: 5'- cUCGAAGUGGGAGCGCUCAUGCGCACAc -3' miRNA: 3'- -AGCUUCACCCUCGCGAGUACGCGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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