Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1053 | 3' | -54.3 | NC_000929.1 | + | 19052 | 0.66 | 0.544344 |
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Target: 5'- --aGGCAGCAGUgaagGCGCUCGGCa---- -3' miRNA: 3'- uuaUUGUCGUCG----CGCGGGUCGgaaaa -5' |
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| 1053 | 3' | -54.3 | NC_000929.1 | + | 2361 | 0.66 | 0.532841 |
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Target: 5'- -cUGACGGgaGGCGCGCCCAaucGCUa--- -3' miRNA: 3'- uuAUUGUCg-UCGCGCGGGU---CGGaaaa -5' |
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| 1053 | 3' | -54.3 | NC_000929.1 | + | 1854 | 0.68 | 0.444618 |
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Target: 5'- --cAAguGCAGC-CGCCCAGUCa--- -3' miRNA: 3'- uuaUUguCGUCGcGCGGGUCGGaaaa -5' |
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| 1053 | 3' | -54.3 | NC_000929.1 | + | 8725 | 0.68 | 0.423848 |
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Target: 5'- cAUGACGGCGGCuuaUGCCCAuGCCa--- -3' miRNA: 3'- uUAUUGUCGUCGc--GCGGGU-CGGaaaa -5' |
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| 1053 | 3' | -54.3 | NC_000929.1 | + | 11097 | 0.69 | 0.36519 |
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Target: 5'- cGUGGCGGguGC-UGUCCAGCCUg-- -3' miRNA: 3'- uUAUUGUCguCGcGCGGGUCGGAaaa -5' |
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| 1053 | 3' | -54.3 | NC_000929.1 | + | 11587 | 0.7 | 0.304166 |
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Target: 5'- --gAGCAGCAGCGCcaccGCCCGGUUc--- -3' miRNA: 3'- uuaUUGUCGUCGCG----CGGGUCGGaaaa -5' |
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| 1053 | 3' | -54.3 | NC_000929.1 | + | 18372 | 0.72 | 0.224613 |
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Target: 5'- --gGACGGCGGCG-GCCCAGCa---- -3' miRNA: 3'- uuaUUGUCGUCGCgCGGGUCGgaaaa -5' |
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| 1053 | 3' | -54.3 | NC_000929.1 | + | 9590 | 0.73 | 0.206218 |
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Target: 5'- --aAACAuCAGCGCGCgCCAGCCa--- -3' miRNA: 3'- uuaUUGUcGUCGCGCG-GGUCGGaaaa -5' |
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| 1053 | 3' | -54.3 | NC_000929.1 | + | 35484 | 0.73 | 0.200382 |
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Target: 5'- -cUGGCGGCAGCaaGUCCAGCCa--- -3' miRNA: 3'- uuAUUGUCGUCGcgCGGGUCGGaaaa -5' |
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| 1053 | 3' | -54.3 | NC_000929.1 | + | 17530 | 0.77 | 0.110743 |
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Target: 5'- gGAUGACAGCAGCGC-CgCAGCCa--- -3' miRNA: 3'- -UUAUUGUCGUCGCGcGgGUCGGaaaa -5' |
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| 1053 | 3' | -54.3 | NC_000929.1 | + | 5436 | 1.04 | 0.001061 |
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Target: 5'- gAAUAACAGCAGCGCGCCCAGCCUUUUg -3' miRNA: 3'- -UUAUUGUCGUCGCGCGGGUCGGAAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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