Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1053 | 5' | -53 | NC_000929.1 | + | 4129 | 0.66 | 0.718085 |
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Target: 5'- -aGAUUGCGCagaaacCAGGUGCGCU-UCGCAu -3' miRNA: 3'- caUUAGCGCG------GUCUAUGCGAcGGUGU- -5' |
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| 1053 | 5' | -53 | NC_000929.1 | + | 17447 | 0.66 | 0.718085 |
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Target: 5'- cGUGAcCGCGCCAuuccccgaguGAUG-GCUGCgGCGc -3' miRNA: 3'- -CAUUaGCGCGGU----------CUAUgCGACGgUGU- -5' |
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| 1053 | 5' | -53 | NC_000929.1 | + | 29614 | 0.66 | 0.69529 |
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Target: 5'- ----cCaCGCCGGAUACGCUGgaaCGCGc -3' miRNA: 3'- cauuaGcGCGGUCUAUGCGACg--GUGU- -5' |
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| 1053 | 5' | -53 | NC_000929.1 | + | 14977 | 0.66 | 0.69529 |
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Target: 5'- aUGcgCGUGCCGGugGCGCUGCaucauCGCGa -3' miRNA: 3'- cAUuaGCGCGGUCuaUGCGACG-----GUGU- -5' |
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| 1053 | 5' | -53 | NC_000929.1 | + | 23966 | 0.67 | 0.625673 |
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Target: 5'- ---cUUGUGUCAGcgAUGCcGCCACAg -3' miRNA: 3'- cauuAGCGCGGUCuaUGCGaCGGUGU- -5' |
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| 1053 | 5' | -53 | NC_000929.1 | + | 3111 | 0.68 | 0.544855 |
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Target: 5'- ----aCGCGCUGGAaguUGCugaacuGCUGCCGCAg -3' miRNA: 3'- cauuaGCGCGGUCU---AUG------CGACGGUGU- -5' |
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| 1053 | 5' | -53 | NC_000929.1 | + | 25767 | 0.69 | 0.533542 |
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Target: 5'- -gAAagGUGCCGGucGUGCGUUGUCACGu -3' miRNA: 3'- caUUagCGCGGUC--UAUGCGACGGUGU- -5' |
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| 1053 | 5' | -53 | NC_000929.1 | + | 29494 | 0.71 | 0.396557 |
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Target: 5'- -gGcgCGCGCUGGAUucugacACGCUGCCGg- -3' miRNA: 3'- caUuaGCGCGGUCUA------UGCGACGGUgu -5' |
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| 1053 | 5' | -53 | NC_000929.1 | + | 5285 | 0.74 | 0.248389 |
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Target: 5'- -cAAUgGCGCgGGAUAUGCUuucGCCGCAa -3' miRNA: 3'- caUUAgCGCGgUCUAUGCGA---CGGUGU- -5' |
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| 1053 | 5' | -53 | NC_000929.1 | + | 5399 | 1.08 | 0.000951 |
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Target: 5'- gGUAAUCGCGCCAGAUACGCUGCCACAu -3' miRNA: 3'- -CAUUAGCGCGGUCUAUGCGACGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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