Results 81 - 83 of 83 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10536 | 3' | -54.8 | NC_002687.1 | + | 10377 | 0.76 | 0.656184 |
Target: 5'- uGCGGCGACggGgGUGGAGAagGCAGCGg- -3' miRNA: 3'- -UGCCGUUGa-UgUACCUCU--CGUCGCgg -5' |
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10536 | 3' | -54.8 | NC_002687.1 | + | 4376 | 0.66 | 0.983032 |
Target: 5'- cACGGguGCc-CGUGGGGAauguuaaaaauugGUAGCGUCg -3' miRNA: 3'- -UGCCguUGauGUACCUCU-------------CGUCGCGG- -5' |
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10536 | 3' | -54.8 | NC_002687.1 | + | 2655 | 0.72 | 0.854464 |
Target: 5'- uUGGCGGCa--AUGGGGAGguGCGUg -3' miRNA: 3'- uGCCGUUGaugUACCUCUCguCGCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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