Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10540 | 5' | -58 | NC_002687.1 | + | 275827 | 0.66 | 0.937751 |
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Target: 5'- cACCCCUCgauccccacgcgCUCGacCAGCUCGCg--- -3' miRNA: 3'- aUGGGGAG------------GAGCaaGUCGAGCGaccu -5' |
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| 10540 | 5' | -58 | NC_002687.1 | + | 216070 | 0.66 | 0.933092 |
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Target: 5'- gGCCUgcgCCgauGUUCAGC-CGCUGGAg -3' miRNA: 3'- aUGGGga-GGag-CAAGUCGaGCGACCU- -5' |
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| 10540 | 5' | -58 | NC_002687.1 | + | 26840 | 0.67 | 0.923132 |
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Target: 5'- aUGCCCCUCCgccUGUUUaaAGUUUGCaugaUGGAa -3' miRNA: 3'- -AUGGGGAGGa--GCAAG--UCGAGCG----ACCU- -5' |
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| 10540 | 5' | -58 | NC_002687.1 | + | 143002 | 0.67 | 0.912317 |
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Target: 5'- cACCCCUCCUCGauaCAccGCUUGUUGc- -3' miRNA: 3'- aUGGGGAGGAGCaa-GU--CGAGCGACcu -5' |
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| 10540 | 5' | -58 | NC_002687.1 | + | 213704 | 0.67 | 0.908906 |
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Target: 5'- gUGCUCCUCCUCGUUCAccGUgaacagguucaucuuUCGUuuuuuUGGAa -3' miRNA: 3'- -AUGGGGAGGAGCAAGU--CG---------------AGCG-----ACCU- -5' |
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| 10540 | 5' | -58 | NC_002687.1 | + | 199122 | 0.7 | 0.780213 |
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Target: 5'- cUGCCCCUCUgcgcggCGUcUAGCUCGUgugUGGGc -3' miRNA: 3'- -AUGGGGAGGa-----GCAaGUCGAGCG---ACCU- -5' |
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| 10540 | 5' | -58 | NC_002687.1 | + | 195113 | 1.02 | 0.012151 |
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Target: 5'- gUACCCCUUCUCGUUCAGCUCGCUGGAc -3' miRNA: 3'- -AUGGGGAGGAGCAAGUCGAGCGACCU- -5' |
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| 10540 | 5' | -58 | NC_002687.1 | + | 19278 | 1.07 | 0.00568 |
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Target: 5'- gUACCCCUCCUCGUUCAGCUCGCUGGAc -3' miRNA: 3'- -AUGGGGAGGAGCAAGUCGAGCGACCU- -5' |
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| 10540 | 5' | -58 | NC_002687.1 | + | 220704 | 1.07 | 0.00568 |
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Target: 5'- gUACCCCUCCUCGUUCAGCUCGCUGGAc -3' miRNA: 3'- -AUGGGGAGGAGCAAGUCGAGCGACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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