Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1055 | 5' | -58.4 | NC_000929.1 | + | 9674 | 0.66 | 0.384523 |
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Target: 5'- uUCAGGCacccgGCAGCUCAUuaucagcgcauccaGACaaucaGCGGCa -3' miRNA: 3'- uAGUCCGg----CGUCGAGUG--------------CUGg----CGUUG- -5' |
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| 1055 | 5' | -58.4 | NC_000929.1 | + | 22936 | 0.66 | 0.369133 |
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Target: 5'- gGUCAGGCCGUGGUg-ACGcauCCGUGAUc -3' miRNA: 3'- -UAGUCCGGCGUCGagUGCu--GGCGUUG- -5' |
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| 1055 | 5' | -58.4 | NC_000929.1 | + | 7248 | 0.67 | 0.318264 |
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Target: 5'- -gCAGGuauCCGCgaAGUUCugGGCCuGCAACu -3' miRNA: 3'- uaGUCC---GGCG--UCGAGugCUGG-CGUUG- -5' |
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| 1055 | 5' | -58.4 | NC_000929.1 | + | 16677 | 0.68 | 0.284428 |
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Target: 5'- -gCGGGCCGCAGacagcgcccggaCGCGACaGCGGCa -3' miRNA: 3'- uaGUCCGGCGUCga----------GUGCUGgCGUUG- -5' |
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| 1055 | 5' | -58.4 | NC_000929.1 | + | 8326 | 0.69 | 0.252161 |
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Target: 5'- aAUCAGuuGCCGCgAGCUgACGGuCaCGCAACu -3' miRNA: 3'- -UAGUC--CGGCG-UCGAgUGCU-G-GCGUUG- -5' |
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| 1055 | 5' | -58.4 | NC_000929.1 | + | 8767 | 0.71 | 0.182066 |
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Target: 5'- cGUCAuGGCaucauagccgCGCAGUUCACGACCG-GACg -3' miRNA: 3'- -UAGU-CCG----------GCGUCGAGUGCUGGCgUUG- -5' |
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| 1055 | 5' | -58.4 | NC_000929.1 | + | 3096 | 0.72 | 0.158321 |
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Target: 5'- uUCuGGCgGCGGCgauauUCACGgccuGCCGCAGCa -3' miRNA: 3'- uAGuCCGgCGUCG-----AGUGC----UGGCGUUG- -5' |
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| 1055 | 5' | -58.4 | NC_000929.1 | + | 13108 | 0.75 | 0.10006 |
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Target: 5'- -gCAGGCCGCAaugUCAUGACgGCAGCg -3' miRNA: 3'- uaGUCCGGCGUcg-AGUGCUGgCGUUG- -5' |
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| 1055 | 5' | -58.4 | NC_000929.1 | + | 11687 | 1.06 | 0.000359 |
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Target: 5'- cAUCAGGCCGCAGCUCACGACCGCAACc -3' miRNA: 3'- -UAGUCCGGCGUCGAGUGCUGGCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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