Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10554 | 5' | -49.3 | NC_002687.1 | + | 238943 | 0.66 | 0.999954 |
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Target: 5'- gGGGAGUcUuuGGUAUCGUUGgaCUGUa- -3' miRNA: 3'- -CCUUCAaAggCCAUGGUAAC--GACGau -5' |
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| 10554 | 5' | -49.3 | NC_002687.1 | + | 279637 | 0.66 | 0.999867 |
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Target: 5'- uGGGccGGUggugCCGGcACCAccUGCUGCUc -3' miRNA: 3'- -CCU--UCAaa--GGCCaUGGUa-ACGACGAu -5' |
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| 10554 | 5' | -49.3 | NC_002687.1 | + | 269100 | 0.67 | 0.999783 |
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Target: 5'- aGGAuGUgagUCgGGUACgGUUGuCUGCa- -3' miRNA: 3'- -CCUuCAa--AGgCCAUGgUAAC-GACGau -5' |
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| 10554 | 5' | -49.3 | NC_002687.1 | + | 175592 | 0.68 | 0.99947 |
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Target: 5'- uGGAuc--UCCGGgcACCAUUGCUGUc- -3' miRNA: 3'- -CCUucaaAGGCCa-UGGUAACGACGau -5' |
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| 10554 | 5' | -49.3 | NC_002687.1 | + | 24042 | 0.69 | 0.998299 |
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Target: 5'- gGGggGUgugCaGGUGCCGcuacuucUGCUGCUGg -3' miRNA: 3'- -CCuuCAaa-GgCCAUGGUa------ACGACGAU- -5' |
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| 10554 | 5' | -49.3 | NC_002687.1 | + | 279358 | 0.7 | 0.996088 |
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Target: 5'- uGGAGUgccgUCCGGUuCCAcUGCcGCUGg -3' miRNA: 3'- cCUUCAa---AGGCCAuGGUaACGaCGAU- -5' |
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| 10554 | 5' | -49.3 | NC_002687.1 | + | 226783 | 0.73 | 0.971043 |
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Target: 5'- uGGAGGUggugCCGGUGCCggUGCcggUGCc- -3' miRNA: 3'- -CCUUCAaa--GGCCAUGGuaACG---ACGau -5' |
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| 10554 | 5' | -49.3 | NC_002687.1 | + | 96669 | 0.74 | 0.957857 |
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Target: 5'- gGGAGGUUUCCGGU-CC-UUGCguaGCg- -3' miRNA: 3'- -CCUUCAAAGGCCAuGGuAACGa--CGau -5' |
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| 10554 | 5' | -49.3 | NC_002687.1 | + | 309198 | 0.75 | 0.925956 |
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Target: 5'- cGGGAGgccUCCGGUGgCAUcgaUGCUGCg- -3' miRNA: 3'- -CCUUCaa-AGGCCAUgGUA---ACGACGau -5' |
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| 10554 | 5' | -49.3 | NC_002687.1 | + | 257091 | 1.1 | 0.020299 |
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Target: 5'- aGGAAGUUUCCGGUACCAUUGCUGCUAg -3' miRNA: 3'- -CCUUCAAAGGCCAUGGUAACGACGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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