Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1056 | 3' | -55.1 | NC_000929.1 | + | 25813 | 0.66 | 0.604637 |
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Target: 5'- gGGGCACu-CCagCuUGCCccCGUCCUGAu -3' miRNA: 3'- -CCCGUGcuGGaaG-ACGGu-GUAGGACU- -5' |
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| 1056 | 3' | -55.1 | NC_000929.1 | + | 33545 | 0.66 | 0.593324 |
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Target: 5'- uGGauuCGACCUUCUGCgcguagaGCAUCCa-- -3' miRNA: 3'- cCCgu-GCUGGAAGACGg------UGUAGGacu -5' |
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| 1056 | 3' | -55.1 | NC_000929.1 | + | 16596 | 0.66 | 0.582046 |
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Target: 5'- uGGCugGAaCUgccgCUGUCGCGUCCgGGc -3' miRNA: 3'- cCCGugCUgGAa---GACGGUGUAGGaCU- -5' |
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| 1056 | 3' | -55.1 | NC_000929.1 | + | 19741 | 0.67 | 0.535264 |
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Target: 5'- cGGGUACGugCgaccaguuccggUUUGCgGCGcUCCUGGa -3' miRNA: 3'- -CCCGUGCugGa-----------AGACGgUGU-AGGACU- -5' |
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| 1056 | 3' | -55.1 | NC_000929.1 | + | 9747 | 0.68 | 0.504814 |
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Target: 5'- uGGGCGCuGGCCggaUUGCUgACGUUCUGGc -3' miRNA: 3'- -CCCGUG-CUGGaa-GACGG-UGUAGGACU- -5' |
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| 1056 | 3' | -55.1 | NC_000929.1 | + | 12291 | 1.11 | 0.000425 |
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Target: 5'- cGGGCACGACCUUCUGCCACAUCCUGAc -3' miRNA: 3'- -CCCGUGCUGGAAGACGGUGUAGGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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