Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10570 | 3' | -49.3 | NC_002687.1 | + | 241164 | 0.66 | 0.999958 |
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Target: 5'- -aCGUCGUGugGCCGAgcaucucUGAUuuuCUUc -3' miRNA: 3'- gaGUAGCGCugCGGCUa------ACUAuu-GAG- -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 232219 | 0.66 | 0.999958 |
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Target: 5'- -cUAUCG-GAUGCCGAacggcgagGGUGACUCc -3' miRNA: 3'- gaGUAGCgCUGCGGCUaa------CUAUUGAG- -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 297440 | 0.66 | 0.999968 |
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Target: 5'- gCUCAUCGCGACGCacaa-GGUcAGCg- -3' miRNA: 3'- -GAGUAGCGCUGCGgcuaaCUA-UUGag -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 69882 | 0.66 | 0.999968 |
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Target: 5'- -cCAUCGCGAcauuguuguCGCCGAcgucGGUGACg- -3' miRNA: 3'- gaGUAGCGCU---------GCGGCUaa--CUAUUGag -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 263017 | 0.66 | 0.999968 |
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Target: 5'- ----cCGCGACGUCGuuGUUGGUGGC-Cg -3' miRNA: 3'- gaguaGCGCUGCGGC--UAACUAUUGaG- -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 83593 | 0.66 | 0.999976 |
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Target: 5'- -aCggCGCGACGCCu-UUGAUuAACUUu -3' miRNA: 3'- gaGuaGCGCUGCGGcuAACUA-UUGAG- -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 180630 | 0.66 | 0.999976 |
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Target: 5'- --gGUCGCGAUGCUc--UGaAUGACUCg -3' miRNA: 3'- gagUAGCGCUGCGGcuaAC-UAUUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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