Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10570 | 3' | -49.3 | NC_002687.1 | + | 251990 | 0.67 | 0.999844 |
|
Target: 5'- ----gCGCGGCGCCGAggGGUAccgGCg- -3' miRNA: 3'- gaguaGCGCUGCGGCUaaCUAU---UGag -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 263017 | 0.66 | 0.999968 |
|
Target: 5'- ----cCGCGACGUCGuuGUUGGUGGC-Cg -3' miRNA: 3'- gaguaGCGCUGCGGC--UAACUAUUGaG- -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 297393 | 0.99 | 0.110824 |
|
Target: 5'- gCUCAUCGCGAC-CCGAUUGAUAACUCg -3' miRNA: 3'- -GAGUAGCGCUGcGGCUAACUAUUGAG- -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 297440 | 0.66 | 0.999968 |
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Target: 5'- gCUCAUCGCGACGCacaa-GGUcAGCg- -3' miRNA: 3'- -GAGUAGCGCUGCGgcuaaCUA-UUGag -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 297662 | 0.74 | 0.970426 |
|
Target: 5'- uUCA-CGCGACGCCGAUcUGAUucAGCg- -3' miRNA: 3'- gAGUaGCGCUGCGGCUA-ACUA--UUGag -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 305168 | 0.67 | 0.999905 |
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Target: 5'- --aGUUGUGGCGUCGGUUGGgcgGACg- -3' miRNA: 3'- gagUAGCGCUGCGGCUAACUa--UUGag -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 307106 | 0.69 | 0.999113 |
|
Target: 5'- gCUCGUC-CGAagcuCGCCGAUUGuuuuccaagaAUGGCUCc -3' miRNA: 3'- -GAGUAGcGCU----GCGGCUAAC----------UAUUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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