Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10570 | 3' | -49.3 | NC_002687.1 | + | 84366 | 0.74 | 0.973206 |
|
Target: 5'- -aCGUCGCGACGaCGGUgacgGAUAACcUCa -3' miRNA: 3'- gaGUAGCGCUGCgGCUAa---CUAUUG-AG- -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 83593 | 0.66 | 0.999976 |
|
Target: 5'- -aCggCGCGACGCCu-UUGAUuAACUUu -3' miRNA: 3'- gaGuaGCGCUGCGGcuAACUA-UUGAG- -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 77561 | 0.66 | 0.999927 |
|
Target: 5'- -gCAUUGCGAgGCCG--UGAUGAUg- -3' miRNA: 3'- gaGUAGCGCUgCGGCuaACUAUUGag -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 69882 | 0.66 | 0.999968 |
|
Target: 5'- -cCAUCGCGAcauuguuguCGCCGAcgucGGUGACg- -3' miRNA: 3'- gaGUAGCGCU---------GCGGCUaa--CUAUUGag -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 57136 | 0.67 | 0.999844 |
|
Target: 5'- -gCAUCgGCGGCGCUGAUggccaUGAguugAGCUUc -3' miRNA: 3'- gaGUAG-CGCUGCGGCUA-----ACUa---UUGAG- -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 34801 | 0.71 | 0.994399 |
|
Target: 5'- uCUC--CGUGACGCCGA--GAUAugUCa -3' miRNA: 3'- -GAGuaGCGCUGCGGCUaaCUAUugAG- -5' |
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| 10570 | 3' | -49.3 | NC_002687.1 | + | 23810 | 0.71 | 0.994399 |
|
Target: 5'- gCUCAa-GCGAgGCCGAUUG-UAACUa -3' miRNA: 3'- -GAGUagCGCUgCGGCUAACuAUUGAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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