miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10572 5' -44.8 NC_002687.1 + 151445 0.66 1
Target:  5'- gCCCGUGugCgaaGAAcgGAGUGUGccuggaUCGGa -3'
miRNA:   3'- -GGGCACugGaa-CUU--UUUACACa-----AGCU- -5'
10572 5' -44.8 NC_002687.1 + 111371 0.66 1
Target:  5'- aCCGgcgucGACUUUGAAAAA-GUGUUUa- -3'
miRNA:   3'- gGGCa----CUGGAACUUUUUaCACAAGcu -5'
10572 5' -44.8 NC_002687.1 + 10686 0.67 1
Target:  5'- aCCCcUGACCacgUGAAGcAcgGUGUUCu- -3'
miRNA:   3'- -GGGcACUGGa--ACUUU-UuaCACAAGcu -5'
10572 5' -44.8 NC_002687.1 + 157700 0.66 1
Target:  5'- uUCCGUGGCCUccUGGAuugcaAAAUGcUGUcUGAu -3'
miRNA:   3'- -GGGCACUGGA--ACUU-----UUUAC-ACAaGCU- -5'
10572 5' -44.8 NC_002687.1 + 300890 0.68 1
Target:  5'- -aCGUGACCUUGGugcgcggugAGAGUGUGgcaacauccgacggaUCGAa -3'
miRNA:   3'- ggGCACUGGAACU---------UUUUACACa--------------AGCU- -5'
10572 5' -44.8 NC_002687.1 + 213384 0.68 1
Target:  5'- gCUGgaaagGGCuCUUGguGAAUGUGUUCGc -3'
miRNA:   3'- gGGCa----CUG-GAACuuUUUACACAAGCu -5'
10572 5' -44.8 NC_002687.1 + 138925 0.68 1
Target:  5'- gCCCGUGgacgucauuuuggaaGCCUUGAuuGAUGUuccaucgUCGGg -3'
miRNA:   3'- -GGGCAC---------------UGGAACUuuUUACAca-----AGCU- -5'
10572 5' -44.8 NC_002687.1 + 77551 0.67 1
Target:  5'- gCCGUGAUgaUGAaguacaccgaauucGAAGUGUGcUCGGa -3'
miRNA:   3'- gGGCACUGgaACU--------------UUUUACACaAGCU- -5'
10572 5' -44.8 NC_002687.1 + 124241 0.66 1
Target:  5'- uUCCGUGACCUuccgcuucuUGAAAAAaGUGg---- -3'
miRNA:   3'- -GGGCACUGGA---------ACUUUUUaCACaagcu -5'
10572 5' -44.8 NC_002687.1 + 221805 0.69 0.999999
Target:  5'- aCCGcaucauUGACCUUGGacuGAAAUGUGUcgcguuucucaUCGGu -3'
miRNA:   3'- gGGC------ACUGGAACU---UUUUACACA-----------AGCU- -5'
10572 5' -44.8 NC_002687.1 + 213955 0.69 0.999999
Target:  5'- cUCCGUGACCUUGcu-GAUGUccagcuucUCGAu -3'
miRNA:   3'- -GGGCACUGGAACuuuUUACAca------AGCU- -5'
10572 5' -44.8 NC_002687.1 + 300563 1.14 0.047805
Target:  5'- cCCCGUGACCUUGAAAAAUGUGUUCGAg -3'
miRNA:   3'- -GGGCACUGGAACUUUUUACACAAGCU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.