Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10574 | 3' | -53.3 | NC_002687.1 | + | 195945 | 0.66 | 0.995818 |
Target: 5'- cCGUCGCCAUacgcugUGCCUcgugcucUCGUUGuugGUCa -3' miRNA: 3'- -GCAGUGGUG------ACGGA-------AGCAACua-CGGc -5' |
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10574 | 3' | -53.3 | NC_002687.1 | + | 163986 | 0.66 | 0.9974 |
Target: 5'- uGUCGCCA---UCUUCGUcGGUGUCGa -3' miRNA: 3'- gCAGUGGUgacGGAAGCAaCUACGGC- -5' |
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10574 | 3' | -53.3 | NC_002687.1 | + | 224259 | 0.66 | 0.997789 |
Target: 5'- gCGaUGCUACUGCCgaUGccGAUGCCGa -3' miRNA: 3'- -GCaGUGGUGACGGaaGCaaCUACGGC- -5' |
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10574 | 3' | -53.3 | NC_002687.1 | + | 280690 | 0.66 | 0.997789 |
Target: 5'- aGUUcCC-CUGCCg-CG-UGAUGCCGg -3' miRNA: 3'- gCAGuGGuGACGGaaGCaACUACGGC- -5' |
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10574 | 3' | -53.3 | NC_002687.1 | + | 307845 | 0.66 | 0.997789 |
Target: 5'- uCGUCAuCCAgUGCCccugUGUUGgcGCCc -3' miRNA: 3'- -GCAGU-GGUgACGGaa--GCAACuaCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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