Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10574 | 3' | -53.3 | NC_002687.1 | + | 44357 | 0.73 | 0.883031 |
Target: 5'- cCGUCugCGCcgGCCUcCGUcGAUGCUGu -3' miRNA: 3'- -GCAGugGUGa-CGGAaGCAaCUACGGC- -5' |
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10574 | 3' | -53.3 | NC_002687.1 | + | 313008 | 0.73 | 0.876189 |
Target: 5'- gCGUgACCACUGCCgaaaaCGcaaUUGAUGCUGu -3' miRNA: 3'- -GCAgUGGUGACGGaa---GC---AACUACGGC- -5' |
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10574 | 3' | -53.3 | NC_002687.1 | + | 161973 | 0.74 | 0.822906 |
Target: 5'- uCGUCACUauGCUGgCggagUCGUUGGUGUCGu -3' miRNA: 3'- -GCAGUGG--UGACgGa---AGCAACUACGGC- -5' |
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10574 | 3' | -53.3 | NC_002687.1 | + | 101010 | 0.76 | 0.743205 |
Target: 5'- gCGgCACCGgUGUCgaCGUUGAUGCCGa -3' miRNA: 3'- -GCaGUGGUgACGGaaGCAACUACGGC- -5' |
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10574 | 3' | -53.3 | NC_002687.1 | + | 307339 | 1.09 | 0.012639 |
Target: 5'- uCGUCACCACUGCCUUCGUUGAUGCCGu -3' miRNA: 3'- -GCAGUGGUGACGGAAGCAACUACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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