Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 10576 | 5' | -52.4 | NC_002687.1 | + | 84681 | 0.66 | 0.999652 |
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Target: 5'- -aGUgAuCGUCAUGGccguCGUCGGUGu -3' miRNA: 3'- ggCAgUuGCAGUACCuu--GCAGCCGCc -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 22157 | 0.66 | 0.999572 |
|
Target: 5'- cCCGgCGACGcCAUgcccuguccuugGGAGCGUucaccaggaggUGGCGGa -3' miRNA: 3'- -GGCaGUUGCaGUA------------CCUUGCA-----------GCCGCC- -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 237448 | 0.66 | 0.999572 |
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Target: 5'- gCGUCGACacccUCAgauguaucaagGcGGACGUCGGCGa -3' miRNA: 3'- gGCAGUUGc---AGUa----------C-CUUGCAGCCGCc -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 248101 | 0.66 | 0.999572 |
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Target: 5'- gCCGagCAACGaCuggGGGGCGUuugacuUGGCGGg -3' miRNA: 3'- -GGCa-GUUGCaGua-CCUUGCA------GCCGCC- -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 46910 | 0.66 | 0.999476 |
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Target: 5'- gCGUUGAUGUCuUGGGugcAUGUCGGaGGc -3' miRNA: 3'- gGCAGUUGCAGuACCU---UGCAGCCgCC- -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 225216 | 0.66 | 0.999362 |
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Target: 5'- aCCGUgcCGGCGcCGcc--GCGUCGGCGGc -3' miRNA: 3'- -GGCA--GUUGCaGUaccuUGCAGCCGCC- -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 297347 | 0.66 | 0.999362 |
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Target: 5'- aCCGagGACGcgcgCgAUGG--UGUCGGCGGa -3' miRNA: 3'- -GGCagUUGCa---G-UACCuuGCAGCCGCC- -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 171073 | 0.66 | 0.999652 |
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Target: 5'- cCCGgagaggaCGGCGUaGUGGAGCGgguaGGCGu -3' miRNA: 3'- -GGCa------GUUGCAgUACCUUGCag--CCGCc -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 258254 | 0.66 | 0.999572 |
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Target: 5'- aCCGUgGagauucGCGUCAcGGAcagcgGCGUCGGUc- -3' miRNA: 3'- -GGCAgU------UGCAGUaCCU-----UGCAGCCGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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