Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10576 | 5' | -52.4 | NC_002687.1 | + | 309547 | 1.13 | 0.011837 |
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Target: 5'- cCCGUCAACGUCAUGGAACGUCGGCGGu -3' miRNA: 3'- -GGCAGUUGCAGUACCUUGCAGCCGCC- -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 215393 | 0.74 | 0.90659 |
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Target: 5'- gCGUgGACGUC-UGGAAacaGUgCGGCGGc -3' miRNA: 3'- gGCAgUUGCAGuACCUUg--CA-GCCGCC- -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 57119 | 0.73 | 0.950456 |
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Target: 5'- cCCGUCAACGggAUGcuGCaUCGGCGGc -3' miRNA: 3'- -GGCAGUUGCagUACcuUGcAGCCGCC- -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 123081 | 0.73 | 0.950456 |
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Target: 5'- aCGUCGACGUCAcGGA-CGggaGGgGGg -3' miRNA: 3'- gGCAGUUGCAGUaCCUuGCag-CCgCC- -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 280918 | 0.71 | 0.974048 |
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Target: 5'- gCC-UCAGCGaugCcgGGGgcccguguugaagcaGCGUCGGCGGg -3' miRNA: 3'- -GGcAGUUGCa--GuaCCU---------------UGCAGCCGCC- -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 84359 | 0.7 | 0.981951 |
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Target: 5'- aCCGUCAACGUCGc--GACGaCGGUGa -3' miRNA: 3'- -GGCAGUUGCAGUaccUUGCaGCCGCc -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 95020 | 0.7 | 0.981951 |
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Target: 5'- --cUCAAaGUCAUGGGcucaAUGUUGGCGGa -3' miRNA: 3'- ggcAGUUgCAGUACCU----UGCAGCCGCC- -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 226951 | 0.7 | 0.989742 |
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Target: 5'- uCCGUCGGCugccgGUgGUGG--UGUCGGUGGc -3' miRNA: 3'- -GGCAGUUG-----CAgUACCuuGCAGCCGCC- -5' |
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| 10576 | 5' | -52.4 | NC_002687.1 | + | 258254 | 0.66 | 0.999572 |
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Target: 5'- aCCGUgGagauucGCGUCAcGGAcagcgGCGUCGGUc- -3' miRNA: 3'- -GGCAgU------UGCAGUaCCU-----UGCAGCCGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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