Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10579 | 3' | -59.9 | NC_002687.1 | + | 60250 | 0.67 | 0.87402 |
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Target: 5'- uUUGCCGCGACauccugcgacgaUCCCU-CUCAGCGa- -3' miRNA: 3'- uGGCGGCGCUG------------AGGGAcGAGUUGCcu -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 19967 | 0.69 | 0.746495 |
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Target: 5'- cACUGCgGUG-CUaCCCauuUGCUCAACGGGg -3' miRNA: 3'- -UGGCGgCGCuGA-GGG---ACGAGUUGCCU- -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 18584 | 0.66 | 0.910861 |
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Target: 5'- cGCCgguGCUGCGACggugucgUCCUGCUguguugaAACGGAa -3' miRNA: 3'- -UGG---CGGCGCUGa------GGGACGAg------UUGCCU- -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 18119 | 0.67 | 0.880644 |
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Target: 5'- aGCCGCUGCGGCUgCaacGCUUAGCa-- -3' miRNA: 3'- -UGGCGGCGCUGAgGga-CGAGUUGccu -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 12530 | 0.69 | 0.772938 |
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Target: 5'- uGCCGCUGCuGCUCCUucUGCcgaGAUGGAg -3' miRNA: 3'- -UGGCGGCGcUGAGGG--ACGag-UUGCCU- -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 11857 | 0.69 | 0.772938 |
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Target: 5'- aGCCGCCGCuGCUacgaccgcuacCCCUGCUaCAACa-- -3' miRNA: 3'- -UGGCGGCGcUGA-----------GGGACGA-GUUGccu -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 9017 | 0.69 | 0.761587 |
|
Target: 5'- cCCGCUGCGGgcauguggauuauuCUCCCUGUUCccccCGGGa -3' miRNA: 3'- uGGCGGCGCU--------------GAGGGACGAGuu--GCCU- -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 6370 | 0.74 | 0.504822 |
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Target: 5'- gACCGCUGCGAgacaugagCCUUGCugUCAGCGGAu -3' miRNA: 3'- -UGGCGGCGCUga------GGGACG--AGUUGCCU- -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 5546 | 0.7 | 0.737499 |
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Target: 5'- gACCGCUGCGAgacaugagUCUUGCugUCAGCGGAu -3' miRNA: 3'- -UGGCGGCGCUga------GGGACG--AGUUGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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