Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10579 | 3' | -59.9 | NC_002687.1 | + | 79922 | 0.7 | 0.737499 |
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Target: 5'- cGCUaCCG-GAgUCCCUGCUgAACGGGa -3' miRNA: 3'- -UGGcGGCgCUgAGGGACGAgUUGCCU- -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 200243 | 0.7 | 0.728425 |
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Target: 5'- gACCG-CGCGGCUCUUgcggUGCUCAAUGuGAa -3' miRNA: 3'- -UGGCgGCGCUGAGGG----ACGAGUUGC-CU- -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 273792 | 0.7 | 0.71007 |
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Target: 5'- cAUUGCCGUuGCUUCCUGUaucucaguauuuUCAGCGGAg -3' miRNA: 3'- -UGGCGGCGcUGAGGGACG------------AGUUGCCU- -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 71951 | 0.7 | 0.71007 |
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Target: 5'- cGCCGCCGgaGAUUCUCucgUGCUUGACGGc -3' miRNA: 3'- -UGGCGGCg-CUGAGGG---ACGAGUUGCCu -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 167890 | 0.71 | 0.644417 |
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Target: 5'- cGuuGCCGCGaaGCUUCCgcgGCgCAACGGAu -3' miRNA: 3'- -UggCGGCGC--UGAGGGa--CGaGUUGCCU- -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 214896 | 0.73 | 0.568974 |
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Target: 5'- cGCCGCCGCcucUUUCCUGUaCGGCGGAa -3' miRNA: 3'- -UGGCGGCGcu-GAGGGACGaGUUGCCU- -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 6370 | 0.74 | 0.504822 |
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Target: 5'- gACCGCUGCGAgacaugagCCUUGCugUCAGCGGAu -3' miRNA: 3'- -UGGCGGCGCUga------GGGACG--AGUUGCCU- -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 308885 | 0.74 | 0.469578 |
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Target: 5'- --aGCgGgGGCUCCCUGCUCGGcCGGGg -3' miRNA: 3'- uggCGgCgCUGAGGGACGAGUU-GCCU- -5' |
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| 10579 | 3' | -59.9 | NC_002687.1 | + | 312879 | 1.07 | 0.003975 |
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Target: 5'- uACCGCCGCGACUCCCUGCUCAACGGAc -3' miRNA: 3'- -UGGCGGCGCUGAGGGACGAGUUGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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