Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10579 | 5' | -59.1 | NC_002687.1 | + | 316222 | 0.66 | 0.912557 |
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Target: 5'- gGAUCCGgcgcgGuGGGaucuuCGGGUGCgGCGGGa -3' miRNA: 3'- -CUAGGCa----CuCCC-----GUCUACGgCGCCUa -5' |
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| 10579 | 5' | -59.1 | NC_002687.1 | + | 281174 | 0.68 | 0.838961 |
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Target: 5'- ---gCGUGGGGGCcGAgGCgGCGGAa -3' miRNA: 3'- cuagGCACUCCCGuCUaCGgCGCCUa -5' |
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| 10579 | 5' | -59.1 | NC_002687.1 | + | 117861 | 0.69 | 0.789912 |
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Target: 5'- aGggUCGUGGGGGUuGAUGCCGaauagaGGAg -3' miRNA: 3'- -CuaGGCACUCCCGuCUACGGCg-----CCUa -5' |
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| 10579 | 5' | -59.1 | NC_002687.1 | + | 118354 | 0.71 | 0.670271 |
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Target: 5'- gGAUCCGUGGGGuGCGGGUuCUGUGGc- -3' miRNA: 3'- -CUAGGCACUCC-CGUCUAcGGCGCCua -5' |
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| 10579 | 5' | -59.1 | NC_002687.1 | + | 270928 | 0.76 | 0.396589 |
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Target: 5'- --aCCGaGuGGGCGGGUGCCGUGGAc -3' miRNA: 3'- cuaGGCaCuCCCGUCUACGGCGCCUa -5' |
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| 10579 | 5' | -59.1 | NC_002687.1 | + | 312913 | 1.06 | 0.004857 |
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Target: 5'- cGAUCCGUGAGGGCAGAUGCCGCGGAUg -3' miRNA: 3'- -CUAGGCACUCCCGUCUACGGCGCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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