Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10582 | 5' | -55.5 | NC_002687.1 | + | 240478 | 0.66 | 0.983723 |
|
Target: 5'- uUUGCAUGCGCUGcacggaucACCGaucuUCCUGAGCu -3' miRNA: 3'- cAGCGUGUGCGAC--------UGGU----GGGACUUGu -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 247185 | 1.08 | 0.007944 |
|
Target: 5'- uGUCGCACACGCUGACCACCCUGAACAu -3' miRNA: 3'- -CAGCGUGUGCGACUGGUGGGACUUGU- -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 253610 | 0.7 | 0.90471 |
|
Target: 5'- -cUGCGCGCGUUGACCGCgaUaGAACAg -3' miRNA: 3'- caGCGUGUGCGACUGGUGggA-CUUGU- -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 253798 | 0.67 | 0.970059 |
|
Target: 5'- aUC-CugGCGCUGGCCcgaACCCUGuuggGCGa -3' miRNA: 3'- cAGcGugUGCGACUGG---UGGGACu---UGU- -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 257696 | 0.66 | 0.983723 |
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Target: 5'- aUCGCG-GCGCUGuCaGCCUUGAACGc -3' miRNA: 3'- cAGCGUgUGCGACuGgUGGGACUUGU- -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 271190 | 0.68 | 0.949676 |
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Target: 5'- -cCGCGCACuCuUGGCCagaGCUCUGAACAu -3' miRNA: 3'- caGCGUGUGcG-ACUGG---UGGGACUUGU- -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 300635 | 0.66 | 0.977653 |
|
Target: 5'- uGUCGCGCuCGCUucaaucguaGCCACCC-GAGCc -3' miRNA: 3'- -CAGCGUGuGCGAc--------UGGUGGGaCUUGu -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 309145 | 0.68 | 0.953585 |
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Target: 5'- cGUC-CAcCACGCUcACgACCCUGAGCc -3' miRNA: 3'- -CAGcGU-GUGCGAcUGgUGGGACUUGu -5' |
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| 10582 | 5' | -55.5 | NC_002687.1 | + | 318730 | 1.08 | 0.007944 |
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Target: 5'- uGUCGCACACGCUGACCACCCUGAACAu -3' miRNA: 3'- -CAGCGUGUGCGACUGGUGGGACUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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