Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1059 | 5' | -52.8 | NC_000929.1 | + | 19026 | 1.06 | 0.001218 |
Target: 5'- gGUCUGCAUGGCAGAUAACGCAGCGAUg -3' miRNA: 3'- -CAGACGUACCGUCUAUUGCGUCGCUA- -5' |
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1059 | 5' | -52.8 | NC_000929.1 | + | 22924 | 0.75 | 0.211077 |
Target: 5'- uUCUGCGUGauGCGGGUu-CGCAGCGAa -3' miRNA: 3'- cAGACGUAC--CGUCUAuuGCGUCGCUa -5' |
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1059 | 5' | -52.8 | NC_000929.1 | + | 25549 | 0.66 | 0.693426 |
Target: 5'- ---gGCGUGGUgcAGGUAAUGCcAGCGGc -3' miRNA: 3'- cagaCGUACCG--UCUAUUGCG-UCGCUa -5' |
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1059 | 5' | -52.8 | NC_000929.1 | + | 13371 | 0.68 | 0.564057 |
Target: 5'- -cCUGCGUGGUuugcaGGggGACGUGGUGAUu -3' miRNA: 3'- caGACGUACCG-----UCuaUUGCGUCGCUA- -5' |
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1059 | 5' | -52.8 | NC_000929.1 | + | 31005 | 0.67 | 0.622793 |
Target: 5'- -aCUGC--GGCGGAUGauGCGCGGCa-- -3' miRNA: 3'- caGACGuaCCGUCUAU--UGCGUCGcua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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