Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 10593 | 3' | -59.6 | NC_002687.1 | + | 270406 | 0.66 | 0.906694 |
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Target: 5'- --gGCuuCGGGCGGGGCugcggagggggacaaGGAGACuGUc -3' miRNA: 3'- uugCGugGCCCGCCUCG---------------UCUCUGuCG- -5' |
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| 10593 | 3' | -59.6 | NC_002687.1 | + | 10314 | 0.66 | 0.898418 |
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Target: 5'- --aGCAgCaGuGGCGGcGGCAGuGGCGGCg -3' miRNA: 3'- uugCGUgG-C-CCGCC-UCGUCuCUGUCG- -5' |
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| 10593 | 3' | -59.6 | NC_002687.1 | + | 25413 | 0.66 | 0.885908 |
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Target: 5'- gGAUGCAC--GGCGGuGCAGGaguaccgcuacGACGGCg -3' miRNA: 3'- -UUGCGUGgcCCGCCuCGUCU-----------CUGUCG- -5' |
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| 10593 | 3' | -59.6 | NC_002687.1 | + | 226808 | 0.66 | 0.885908 |
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Target: 5'- --gGUGCCGguGGCGGuGGCGGuGGCGGUg -3' miRNA: 3'- uugCGUGGC--CCGCC-UCGUCuCUGUCG- -5' |
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| 10593 | 3' | -59.6 | NC_002687.1 | + | 309178 | 0.66 | 0.910117 |
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Target: 5'- cGAUGCugC-GGCGGAGacu-GGCGGCg -3' miRNA: 3'- -UUGCGugGcCCGCCUCgucuCUGUCG- -5' |
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| 10593 | 3' | -59.6 | NC_002687.1 | + | 201269 | 0.66 | 0.910681 |
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Target: 5'- gGACgGUACCucgaaaaacaauuauGGCGGcguaaAGCAGAGACGGUa -3' miRNA: 3'- -UUG-CGUGGc--------------CCGCC-----UCGUCUCUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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