Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10593 | 3' | -59.6 | NC_002687.1 | + | 10371 | 0.69 | 0.760835 |
Target: 5'- aGugGCugCGGcGaCGGGGguGgagaAGGCAGCg -3' miRNA: 3'- -UugCGugGCC-C-GCCUCguC----UCUGUCG- -5' |
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10593 | 3' | -59.6 | NC_002687.1 | + | 225582 | 0.69 | 0.778459 |
Target: 5'- --gGgGCCGGaGCGGGGgguggugcUGGAGACGGCg -3' miRNA: 3'- uugCgUGGCC-CGCCUC--------GUCUCUGUCG- -5' |
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10593 | 3' | -59.6 | NC_002687.1 | + | 224907 | 0.69 | 0.778459 |
Target: 5'- gGugGCAUgugGuGGCGGAGgaGGAGACAGUg -3' miRNA: 3'- -UugCGUGg--C-CCGCCUCg-UCUCUGUCG- -5' |
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10593 | 3' | -59.6 | NC_002687.1 | + | 214982 | 0.68 | 0.787102 |
Target: 5'- -uCGaCACCGG-CGGGGCcGAGgGCGGCg -3' miRNA: 3'- uuGC-GUGGCCcGCCUCGuCUC-UGUCG- -5' |
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10593 | 3' | -59.6 | NC_002687.1 | + | 225626 | 0.68 | 0.787102 |
Target: 5'- gGACGCACCgguGGGCGGucgAGCuGGAGcCGGa -3' miRNA: 3'- -UUGCGUGG---CCCGCC---UCG-UCUCuGUCg -5' |
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10593 | 3' | -59.6 | NC_002687.1 | + | 331014 | 1.09 | 0.003046 |
Target: 5'- gAACGCACCGGGCGGAGCAGAGACAGCu -3' miRNA: 3'- -UUGCGUGGCCCGCCUCGUCUCUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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