Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10595 | 5' | -55.7 | NC_002687.1 | + | 295322 | 0.73 | 0.778574 |
Target: 5'- -gCGuGCGAGUCCGCcuacagGCagGCGUUGCUCGa -3' miRNA: 3'- gaGC-UGCUCAGGCG------CG--UGCAACGAGU- -5' |
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10595 | 5' | -55.7 | NC_002687.1 | + | 276134 | 0.75 | 0.647413 |
Target: 5'- gUCGAUGAGcCUGCGCAUGacccUGCUCGa -3' miRNA: 3'- gAGCUGCUCaGGCGCGUGCa---ACGAGU- -5' |
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10595 | 5' | -55.7 | NC_002687.1 | + | 331986 | 0.76 | 0.608651 |
Target: 5'- aUCGAUGAuugGUCCGUGguCGUUGUUCAu -3' miRNA: 3'- gAGCUGCU---CAGGCGCguGCAACGAGU- -5' |
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10595 | 5' | -55.7 | NC_002687.1 | + | 331834 | 1.08 | 0.008348 |
Target: 5'- gCUCGACGAGUCCGCGCACGUUGCUCAu -3' miRNA: 3'- -GAGCUGCUCAGGCGCGUGCAACGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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