miRNA: 3'- gUACCAC---AGCC--UGUAugCGAGAcGCG- -5'
miRNA: 3'- guaCCACAGC-CUgUaUGCGAgACgCG- -5'
miRNA: 3'- guaCCA-CAGCCUguaUGCGA---GACGCG- -5'
miRNA: 3'- gUACCACAGcc--UGUAUgCGaGACGCG- -5'
miRNA: 3'- guaccacAGCCUGUAUGCGAGa--CGCg -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10597 | 3' | -53.2 | NC_002687.1 | + | 109121 | 0.67 | 0.992336 |
|
Target: 5'- gGUGGUGcucUCGGuaAUAUugGCUCUcUGCu -3' miRNA: 3'- gUACCAC---AGCC--UGUAugCGAGAcGCG- -5' |
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| 10597 | 3' | -53.2 | NC_002687.1 | + | 205439 | 0.67 | 0.991278 |
|
Target: 5'- --cGGUGUCGuGGgAcACGCUgUGgGCa -3' miRNA: 3'- guaCCACAGC-CUgUaUGCGAgACgCG- -5' |
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| 10597 | 3' | -53.2 | NC_002687.1 | + | 213627 | 0.67 | 0.991278 |
|
Target: 5'- --gGGUaGUCGGG---ACGCUgucCUGCGCg -3' miRNA: 3'- guaCCA-CAGCCUguaUGCGA---GACGCG- -5' |
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| 10597 | 3' | -53.2 | NC_002687.1 | + | 156859 | 0.7 | 0.954182 |
|
Target: 5'- gGUGGUGUCcauaGCAUGgGUcCUGCGCg -3' miRNA: 3'- gUACCACAGcc--UGUAUgCGaGACGCG- -5' |
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| 10597 | 3' | -53.2 | NC_002687.1 | + | 317199 | 0.66 | 0.997208 |
|
Target: 5'- ------aUCGGGCAUAUGCUCgagGCGg -3' miRNA: 3'- guaccacAGCCUGUAUGCGAGa--CGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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