miRNA: 3'- guACCA---CAGCCUGua--UGCGAGA-----CGCG- -5'
miRNA: 3'- gUACCACAGcc--UGUAUgCGaGACGCG- -5'
miRNA: 3'- -GuaCC-ACAGCCUGUAug-CGA-GAC-GCG- -5'
miRNA: 3'- guaCCACAGc--CUGUaUGCGAgACGCG- -5'
miRNA: 3'- -GUACCACAGCCUGUAUGCGAGACGCG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10597 | 3' | -53.2 | NC_002687.1 | + | 238952 | 0.69 | 0.970609 |
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Target: 5'- -uUGGUaucGUUGGACuguaGCGCUCUcacagGCGCa -3' miRNA: 3'- guACCA---CAGCCUGua--UGCGAGA-----CGCG- -5' |
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| 10597 | 3' | -53.2 | NC_002687.1 | + | 156859 | 0.7 | 0.954182 |
|
Target: 5'- gGUGGUGUCcauaGCAUGgGUcCUGCGCg -3' miRNA: 3'- gUACCACAGcc--UGUAUgCGaGACGCG- -5' |
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| 10597 | 3' | -53.2 | NC_002687.1 | + | 170800 | 0.71 | 0.941807 |
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Target: 5'- aCcgGGcUGUCGGACAUcgaGCUgCUGuCGCg -3' miRNA: 3'- -GuaCC-ACAGCCUGUAug-CGA-GAC-GCG- -5' |
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| 10597 | 3' | -53.2 | NC_002687.1 | + | 133855 | 0.73 | 0.892721 |
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Target: 5'- --cGGUGUCaaaGACG-ACGCUgUGCGCg -3' miRNA: 3'- guaCCACAGc--CUGUaUGCGAgACGCG- -5' |
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| 10597 | 3' | -53.2 | NC_002687.1 | + | 332855 | 1.11 | 0.00943 |
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Target: 5'- gCAUGGUGUCGGACAUACGCUCUGCGCc -3' miRNA: 3'- -GUACCACAGCCUGUAUGCGAGACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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