Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
106 | 3' | -61.9 | AC_000006.1 | + | 13086 | 0.66 | 0.268757 |
Target: 5'- -cGCGGGCguGcGCAcCGCCUCCuggcgcuuuugcaaGGUCg -3' miRNA: 3'- ucCGCUCGguCaCGU-GCGGAGG--------------CCAG- -5' |
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106 | 3' | -61.9 | AC_000006.1 | + | 13130 | 0.67 | 0.226071 |
Target: 5'- aGGGUGAGCCGGc-CGCGCUUgCUGGg- -3' miRNA: 3'- -UCCGCUCGGUCacGUGCGGA-GGCCag -5' |
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106 | 3' | -61.9 | AC_000006.1 | + | 12018 | 0.68 | 0.203425 |
Target: 5'- gAGGcCGGGCUAGUGUacaACGCCcugCUGGa- -3' miRNA: 3'- -UCC-GCUCGGUCACG---UGCGGa--GGCCag -5' |
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106 | 3' | -61.9 | AC_000006.1 | + | 5815 | 0.68 | 0.198079 |
Target: 5'- gGGGCGGGUCuGUGCuCGUCcucacucucuUCCGcGUCg -3' miRNA: 3'- -UCCGCUCGGuCACGuGCGG----------AGGC-CAG- -5' |
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106 | 3' | -61.9 | AC_000006.1 | + | 20809 | 1.08 | 0.000123 |
Target: 5'- aAGGCGAGCCAGUGCACGCCUCCGGUCu -3' miRNA: 3'- -UCCGCUCGGUCACGUGCGGAGGCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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