miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
106 5' -55.1 AC_000006.1 + 20768 1.08 0.000449
Target:  5'- gUUGACAAUGGCGCAGGCCAGCUUGUCg -3'
miRNA:   3'- -AACUGUUACCGCGUCCGGUCGAACAG- -5'
106 5' -55.1 AC_000006.1 + 13334 0.72 0.219792
Target:  5'- -cGGCGcgGGCGCAGGCUcccuaugguccugAGCguagGUCu -3'
miRNA:   3'- aaCUGUuaCCGCGUCCGG-------------UCGaa--CAG- -5'
106 5' -55.1 AC_000006.1 + 5053 0.7 0.31408
Target:  5'- -aGACGAUGGgcguccaGCGcGGCCAGCgucaUGUCc -3'
miRNA:   3'- aaCUGUUACCg------CGU-CCGGUCGa---ACAG- -5'
106 5' -55.1 AC_000006.1 + 14915 0.68 0.414634
Target:  5'- gUGAUggUgGGCGCGGGCggaCGGCagaggaucuggUUGUCg -3'
miRNA:   3'- aACUGuuA-CCGCGUCCG---GUCG-----------AACAG- -5'
106 5' -55.1 AC_000006.1 + 23931 0.66 0.488059
Target:  5'- aUGGCAAgcugccaugGGCGUGGGC-AGCagUGUCu -3'
miRNA:   3'- aACUGUUa--------CCGCGUCCGgUCGa-ACAG- -5'
106 5' -55.1 AC_000006.1 + 7595 0.66 0.521361
Target:  5'- -cGAaaaGAUGGcCGUGGGCCAGCa---- -3'
miRNA:   3'- aaCUg--UUACC-GCGUCCGGUCGaacag -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.