Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
106 | 5' | -55.1 | AC_000006.1 | + | 20768 | 1.08 | 0.000449 |
Target: 5'- gUUGACAAUGGCGCAGGCCAGCUUGUCg -3' miRNA: 3'- -AACUGUUACCGCGUCCGGUCGAACAG- -5' |
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106 | 5' | -55.1 | AC_000006.1 | + | 13334 | 0.72 | 0.219792 |
Target: 5'- -cGGCGcgGGCGCAGGCUcccuaugguccugAGCguagGUCu -3' miRNA: 3'- aaCUGUuaCCGCGUCCGG-------------UCGaa--CAG- -5' |
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106 | 5' | -55.1 | AC_000006.1 | + | 5053 | 0.7 | 0.31408 |
Target: 5'- -aGACGAUGGgcguccaGCGcGGCCAGCgucaUGUCc -3' miRNA: 3'- aaCUGUUACCg------CGU-CCGGUCGa---ACAG- -5' |
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106 | 5' | -55.1 | AC_000006.1 | + | 14915 | 0.68 | 0.414634 |
Target: 5'- gUGAUggUgGGCGCGGGCggaCGGCagaggaucuggUUGUCg -3' miRNA: 3'- aACUGuuA-CCGCGUCCG---GUCG-----------AACAG- -5' |
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106 | 5' | -55.1 | AC_000006.1 | + | 23931 | 0.66 | 0.488059 |
Target: 5'- aUGGCAAgcugccaugGGCGUGGGC-AGCagUGUCu -3' miRNA: 3'- aACUGUUa--------CCGCGUCCGgUCGa-ACAG- -5' |
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106 | 5' | -55.1 | AC_000006.1 | + | 7595 | 0.66 | 0.521361 |
Target: 5'- -cGAaaaGAUGGcCGUGGGCCAGCa---- -3' miRNA: 3'- aaCUg--UUACC-GCGUCCGGUCGaacag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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