miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1062 5' -58.7 NC_000929.1 + 12296 0.68 0.298222
Target:  5'- gAGGCGUgAUGGaCAggaaaacaCGGGGCCGGg- -3'
miRNA:   3'- -UCUGCAgUACC-GUag------GCCCUGGCCgu -5'
1062 5' -58.7 NC_000929.1 + 1931 0.68 0.329336
Target:  5'- -aAUGUUAUGaGCGUCUGGaACUGGCAg -3'
miRNA:   3'- ucUGCAGUAC-CGUAGGCCcUGGCCGU- -5'
1062 5' -58.7 NC_000929.1 + 24970 0.66 0.407789
Target:  5'- uGGcACGaCAUGGCGgauaaaUCCGGGgaacuugacgcACUGGCAg -3'
miRNA:   3'- -UC-UGCaGUACCGU------AGGCCC-----------UGGCCGU- -5'
1062 5' -58.7 NC_000929.1 + 25366 1.08 0.000308
Target:  5'- cAGACGUCAUGGCAUCCGGGACCGGCAg -3'
miRNA:   3'- -UCUGCAGUACCGUAGGCCCUGGCCGU- -5'
1062 5' -58.7 NC_000929.1 + 29347 0.66 0.407789
Target:  5'- uGAUG--AUGGCAUCCGGGGCa-GUAa -3'
miRNA:   3'- uCUGCagUACCGUAGGCCCUGgcCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.