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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 10625 | 5' | -53.6 | NC_002702.1 | + | 15593 | 0.67 | 0.620245 |
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Target: 5'- cGGAAcGGCCCCGAUG-GCCgCG-GCAAu -3' miRNA: 3'- -CCUU-UUGGGGCUGCgCGG-GCaUGUUc -5' |
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| 10625 | 5' | -53.6 | NC_002702.1 | + | 3041 | 0.68 | 0.586063 |
|
Target: 5'- aGAGuGGCCCCGGCucugguGCGUCCGUAUccGa -3' miRNA: 3'- cCUU-UUGGGGCUG------CGCGGGCAUGuuC- -5' |
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| 10625 | 5' | -53.6 | NC_002702.1 | + | 90 | 0.69 | 0.486674 |
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Target: 5'- cGGGGuuuUCCCGgcgggcggGCGCGCCCcguggcGUGCGAGg -3' miRNA: 3'- -CCUUuu-GGGGC--------UGCGCGGG------CAUGUUC- -5' |
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| 10625 | 5' | -53.6 | NC_002702.1 | + | 32532 | 0.69 | 0.486674 |
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Target: 5'- cGGGGuuuUCCCGgcgggcggGCGCGCCCcguggcGUGCGAGg -3' miRNA: 3'- -CCUUuu-GGGGC--------UGCGCGGG------CAUGUUC- -5' |
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| 10625 | 5' | -53.6 | NC_002702.1 | + | 32489 | 1.12 | 0.000558 |
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Target: 5'- gGGAAAACCCCGACGCGCCCGUACAAGg -3' miRNA: 3'- -CCUUUUGGGGCUGCGCGGGCAUGUUC- -5' |
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| 10625 | 5' | -53.6 | NC_002702.1 | + | 133 | 1.12 | 0.000558 |
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Target: 5'- gGGAAAACCCCGACGCGCCCGUACAAGg -3' miRNA: 3'- -CCUUUUGGGGCUGCGCGGGCAUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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