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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10627 | 3' | -58.2 | NC_002702.1 | + | 13876 | 0.68 | 0.300289 |
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Target: 5'- gGGUGGCCAuucgcucggccuccaGGAGcgugcgcgcGGCCagaGCCCUgcugCGGCg -3' miRNA: 3'- aCCACCGGU---------------UCUC---------UCGG---UGGGA----GUCG- -5' |
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| 10627 | 3' | -58.2 | NC_002702.1 | + | 8354 | 0.68 | 0.296461 |
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Target: 5'- cGGaGGCgAAGaAGAGCCGCcuccccuCCUCAGa -3' miRNA: 3'- aCCaCCGgUUC-UCUCGGUG-------GGAGUCg -5' |
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| 10627 | 3' | -58.2 | NC_002702.1 | + | 12657 | 0.69 | 0.24748 |
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Target: 5'- aGGUGGCCAc----GCCACCgCUCuuGGCg -3' miRNA: 3'- aCCACCGGUucucuCGGUGG-GAG--UCG- -5' |
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| 10627 | 3' | -58.2 | NC_002702.1 | + | 7837 | 0.69 | 0.228362 |
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Target: 5'- cGGUGGCCGccAGGuCCACCUgaacgCGGCu -3' miRNA: 3'- aCCACCGGUucUCUcGGUGGGa----GUCG- -5' |
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| 10627 | 3' | -58.2 | NC_002702.1 | + | 10869 | 0.72 | 0.146488 |
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Target: 5'- aUGGUGGCCuuaaacGCCuuCCUCAGCa -3' miRNA: 3'- -ACCACCGGuucucuCGGugGGAGUCG- -5' |
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| 10627 | 3' | -58.2 | NC_002702.1 | + | 13931 | 0.72 | 0.142379 |
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Target: 5'- cUGGaGGCCGAGcGAaugGCCACCCU-GGCg -3' miRNA: 3'- -ACCaCCGGUUCuCU---CGGUGGGAgUCG- -5' |
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| 10627 | 3' | -58.2 | NC_002702.1 | + | 20074 | 0.74 | 0.100706 |
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Target: 5'- gGGUGGUCGAGAGuGGCUaaaACCUUCGGg -3' miRNA: 3'- aCCACCGGUUCUC-UCGG---UGGGAGUCg -5' |
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| 10627 | 3' | -58.2 | NC_002702.1 | + | 7555 | 1.12 | 0.000133 |
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Target: 5'- cUGGUGGCCAAGAGAGCCACCCUCAGCg -3' miRNA: 3'- -ACCACCGGUUCUCUCGGUGGGAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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