miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10628 3' -58.4 NC_002702.1 + 10333 0.68 0.299597
Target:  5'- aCGAGGAGGGGCagggccCGCCUgcgcagccuaCCCCguuccggCCUCg -3'
miRNA:   3'- cGCUUCUUCUCG------GCGGA----------GGGGa------GGAG- -5'
10628 3' -58.4 NC_002702.1 + 11104 0.68 0.298836
Target:  5'- gGCGGAGAGGAguaacaucuccacGCUGCugaCUCCCaacaccaggCUCCUCu -3'
miRNA:   3'- -CGCUUCUUCU-------------CGGCG---GAGGG---------GAGGAG- -5'
10628 3' -58.4 NC_002702.1 + 19026 0.68 0.284665
Target:  5'- uGCGAuGGAGGAGCCuaCUCUgCUCUa- -3'
miRNA:   3'- -CGCU-UCUUCUCGGcgGAGGgGAGGag -5'
10628 3' -58.4 NC_002702.1 + 10392 0.73 0.129075
Target:  5'- uGCGcAGgcGGGCC-CUgCCCCUCCUCg -3'
miRNA:   3'- -CGCuUCuuCUCGGcGGaGGGGAGGAG- -5'
10628 3' -58.4 NC_002702.1 + 8350 1.11 0.000153
Target:  5'- gGCGAAGAAGAGCCGCCUCCCCUCCUCa -3'
miRNA:   3'- -CGCUUCUUCUCGGCGGAGGGGAGGAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.