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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
10628 | 3' | -58.4 | NC_002702.1 | + | 10333 | 0.68 | 0.299597 |
Target: 5'- aCGAGGAGGGGCagggccCGCCUgcgcagccuaCCCCguuccggCCUCg -3' miRNA: 3'- cGCUUCUUCUCG------GCGGA----------GGGGa------GGAG- -5' |
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10628 | 3' | -58.4 | NC_002702.1 | + | 11104 | 0.68 | 0.298836 |
Target: 5'- gGCGGAGAGGAguaacaucuccacGCUGCugaCUCCCaacaccaggCUCCUCu -3' miRNA: 3'- -CGCUUCUUCU-------------CGGCG---GAGGG---------GAGGAG- -5' |
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10628 | 3' | -58.4 | NC_002702.1 | + | 19026 | 0.68 | 0.284665 |
Target: 5'- uGCGAuGGAGGAGCCuaCUCUgCUCUa- -3' miRNA: 3'- -CGCU-UCUUCUCGGcgGAGGgGAGGag -5' |
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10628 | 3' | -58.4 | NC_002702.1 | + | 10392 | 0.73 | 0.129075 |
Target: 5'- uGCGcAGgcGGGCC-CUgCCCCUCCUCg -3' miRNA: 3'- -CGCuUCuuCUCGGcGGaGGGGAGGAG- -5' |
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10628 | 3' | -58.4 | NC_002702.1 | + | 8350 | 1.11 | 0.000153 |
Target: 5'- gGCGAAGAAGAGCCGCCUCCCCUCCUCa -3' miRNA: 3'- -CGCUUCUUCUCGGCGGAGGGGAGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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