Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 10635 | 3' | -57.7 | NC_002702.1 | + | 22957 | 0.66 | 0.416412 |
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Target: 5'- --aGGggCACGGGGUauaccucGACCCaCAGACc -3' miRNA: 3'- aggCCa-GUGCCCCAgu-----UUGGG-GUCUG- -5' |
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| 10635 | 3' | -57.7 | NC_002702.1 | + | 4760 | 0.66 | 0.426055 |
|
Target: 5'- -gUGGaugCugGGGGUCAAAUaCCAGAg -3' miRNA: 3'- agGCCa--GugCCCCAGUUUGgGGUCUg -5' |
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| 10635 | 3' | -57.7 | NC_002702.1 | + | 9452 | 0.66 | 0.434846 |
|
Target: 5'- cUCCGGUCAUcaaugaGGGGgaaggaguggcCAggcuugacgccgaGACCCCAGAa -3' miRNA: 3'- -AGGCCAGUG------CCCCa----------GU-------------UUGGGGUCUg -5' |
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| 10635 | 3' | -57.7 | NC_002702.1 | + | 8232 | 0.66 | 0.465907 |
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Target: 5'- cUUCGaagCugGuGGGUgGAACCUCAGGCu -3' miRNA: 3'- -AGGCca-GugC-CCCAgUUUGGGGUCUG- -5' |
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| 10635 | 3' | -57.7 | NC_002702.1 | + | 12781 | 0.67 | 0.379211 |
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Target: 5'- uUCC-GUC-UGGGGUUuGACCCCGuGACc -3' miRNA: 3'- -AGGcCAGuGCCCCAGuUUGGGGU-CUG- -5' |
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| 10635 | 3' | -57.7 | NC_002702.1 | + | 13981 | 0.68 | 0.31968 |
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Target: 5'- cCCGGUgGCGGauguGGUCAGGgCCgugCAGGCg -3' miRNA: 3'- aGGCCAgUGCC----CCAGUUUgGG---GUCUG- -5' |
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| 10635 | 3' | -57.7 | NC_002702.1 | + | 13845 | 0.68 | 0.335948 |
|
Target: 5'- gUCGGcCACGGGGUCG--UCCguGAUc -3' miRNA: 3'- aGGCCaGUGCCCCAGUuuGGGguCUG- -5' |
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| 10635 | 3' | -57.7 | NC_002702.1 | + | 23024 | 0.69 | 0.274457 |
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Target: 5'- cUCCGGUC-UGuGGGUCGAgguauACCCCGuGCc -3' miRNA: 3'- -AGGCCAGuGC-CCCAGUU-----UGGGGUcUG- -5' |
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| 10635 | 3' | -57.7 | NC_002702.1 | + | 12842 | 1.1 | 0.000235 |
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Target: 5'- aUCCGGUCACGGGGUCAAACCCCAGACg -3' miRNA: 3'- -AGGCCAGUGCCCCAGUUUGGGGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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